1. Immunology of CELIAC DISEASE
Ibrahim, Ibrahim; Kandoth, Littin; Naqvi, Shabab; Sims, Tyreen; Julien, Tabitha
Mentor: Dr. Sanil Manavalan
Columbia University, College of Physicians & Surgeons

2. Celiac disease
a digestive disease that damages the small intestine and interferes with absorption of nutrients from food .
When people with celiac disease eat foods containing gluten, their immune system responds by damaging the small intestine.
Because the body's own immune system causes the damage, celiac disease is considered an autoimmune disorder.

3. AUTOIMMUNE DISEASE: condition in which the immune system mistakenly attacks the body's own organs and tissues
Type 1 Diabetes – affects the pancreas
Systemic Lupus erythmatotosus (Lupus)– affects the skin
Crohn’s Disease- affects the gut
Multiple Sclerosis- affects the brain

4. Key Terms
Cytokine – proteins secreted by macrophages and leukocytes that stimulate or inhibit the proliferation or function of immune cells.
Interleukin – cytokines that only affect leukocytes.
Lymphocyte – A type of leukocyte (white blood cell) of the immune system.
T-Regulatory Cell – slows down and stops immune response
T-Helper Cell – has antigen-receptors, releases cytokines
Natural Killer Cell – kills macrophage and bad stuff
Cytotoxic T-Cell – kills with cytotoxins
B-Cell – creates antibodies.
Antigen – invading microbe
Antibody – protein that fights bacteria and viruses.
Cytokines

5. The Gut Lumen
Villi- (singular: villus) are tiny, finger-like structures that protrude from the wall of the intestine
-Microvilli- hairlike structure on the surface of absorptive and secretory epithelial cells
Epithelial Tissue- layer of cells which line the GI tract
Enterocytes – layer between gut lumen and absorptive cells
diagram

7. LAMINA PROPRIA AND INTRA EPITHILIAL SPACE
GUT LUMEN
GUT LUMEN
Gliadin
Inflammation &
Damaged
enteroctytes
Enterocytes
ab T cell
receptor
Helper T cell
Lymphocytes
(T cells , Natural Killer cells and B cells)
Plasma cells
Antibodies
anti-gliadin
anti-endomysial
And tissue trasgliutaminase
Cytokines
(Interferon-g, TNF-a, IL-15 etc)
HLA-DQ2/DQ8
molecule
Antigen Presenting
Cell
LAMINA PROPRIA AND INTRA EPITHILIAL SPACE

9. Mucosal Immune System

10. Objective
To find the cytokine levels in the serum of patients in the following groups
Healthy Patients (Control)
Active Celiac Disease Patients
Celiac Disease patients on Gluten-free diets
Refractory Disease Patients
The following cytokines were checked in the serum of these patients;
IL-1β
IL-6
IL-8
IL-12 p70
IFN-γ
TNF-α

11. Flow Cytometry DEFINITION:
technique for counting, examining and sorting microscopic particles suspended in a stream of fluid
ROLE IN OUR EXPERIMENT:

13. Results

14. Conclusions

15. References

16. Harlem Children Society
THANK YOU
To: Dr. Sanil Manavalan
Dr. Jamal Naiyer
Sindhu Kunduvaram
Dr. Sat Bhattacharya
Harlem Children Society

17. Cytokines Cytokine Principal cell Targets and biological effects
IL-1β
IL-6
IL-8
IL-12 p70
IFN-γ
TNF-α
Principal cell Targets and biological effects
Endothelial cells: activation
B cells: proliferation of antibody producing cells
Activator of neutrophils
NK cells and T cells: IFN-gamma synthesis, increased cytolytic activity
Macrophages: Activation
Endothelial cells: Activation