1. Michelle Anthony Khadija Lawrence

2. Gram-negative Rod shaped Pathogenic strains http://www.universityofcalifornia.edu/every day/agriculture/images/e_coli.jpg

3. http://upload.wikimedia.org/wikipedia/commons/4/48/Ampicillin-2D-skeletal.png

4. The maximum growth of E. coli will be inhibited as the concentration of ampicillin is increased.

5. Culture (E. coli) Ampicillin 10x lower LB H2O

6. P. fluorescens resistant Titrations No derived conclusion Lead to redesign

7. P. fluorescens resistant Titrations No derived conclusion Lead to redesign

8. P. fluorescens resistant Titrations No derived conclusion Lead to redesign

9. No titrations Altered amounts of ampicillin Varied the ranges each week

10. No titrations Altered amounts of ampicillin Varied the ranges each week Results from each week lead to final experiment

11. LBH2OE. coliAmp 12522410 22522113 32521915 42521519 525212112 625208116 725204120 825199125 * All values in table are in microL

12. 16 12 950 3

13. Maximum growth was not inhibited Normal curve max: 1.088 Highest amp max: 1.044 Lag phase dramatically affected after 16microL is exceeded IC50 not determined Ampicillin affects length of lag phase Graph consistent with scale

14. http://www.cdc.gov/ecoli/general/ http://www.foodsafety.gov/poisoning/causes/bacteria viruses/ecoli/ http://www.foodsafety.gov/poisoning/causes/bacteria viruses/ecoli/ http://www.ncbi.nlm.nih.gov/pmc/articles/PMC15836 96/?page=4 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC15836 96/?page=4