1. Effects of Glucose on the Growth Curve of Pseudomonas fluorescens Muhammad Khan & Andrew Passer November 28, 2011

2. Our Question: What effect does the addition of glucose have on the growth curve of Pseudomonas fluorescens?

3. Interest in Glucose Experiment 2Experiment 3 ??

4. Experiment 4 Supports Experiment 3

5. Is glucose metabolism in P. fluorescens acquired or inherited? We could not “shock” P. fluorescens into increased growth by raising it in a glucose-rich, tryptone-poor environment prior to inoculation.

6. Calculating "Growth Points"

7. What Concentration of Glucose Gives the Highest Level of Growth? Average: 3.418% glucose

8. Conclusion Addition of glucose has a positive effect on the growth of P. fluorescens. Glucose metabolism is an inherited trait for this species. Optimum growth occurs at about 3.4% glucose.

9. Discussion & Future Directions More time needed for P. fluorescens to grow. Is the prolonged increase in OD actual growth? What are the waste products of the different metabolic pathways (amino acid vs. sugar)? How do the waste products affect the stationary phase?

10. Any questions?