1. Field study in Cesena/Italy 2008 Claudio Venturelli, Dipartimento Sanità pubblica, Ausl Cesena, Italy Christian Engelbrecht, University Regensburg Germany Martin Geier, Biogents AG, Regensburg Germany Control of Asian Tiger Mosquitoes by the use of Biogents Mosquito Traps

2. Location: Cesena/Italy, city with 100.000 people Time: June to October 2008 Study design: Selection of 6 areas in Cesena where Asian Tiger mosquitoes (Aedes albopictus) occur. (Based on ovitrap measurements of the last years)‏ 3 areas are randomly allocated to receive the treatment with traps, the other 3 areas are control areas where no traps are placed. The treatment sector receives 8 traps, placed outdoor, run constantly for 24h, from June to October. The control sector has no traps. Following parameters are measured in all sectors: - Human landing rates (same person, 1h in the afternoon, every 5-7 days)‏ - 2 Ovitraps are placed in a sector, weekly collections

3. Intervention and control areas Six areas with simlar environmental conditions have been selected -Three intervention areas with placement of traps: red dots -Three control areas without placement of traps: blue dots

4. Area 1: Treatment with traps 25 m 40m x 45m = 1800sqm M Biogents traps Ovitraps (for monitoring deposited eggs) Position, where human landing rates are measured

5. Area 2: Treatment with traps 60m x 50m = 3000 sqm 20 m M Biogents traps Ovitraps (for monitoring deposited eggs) Position, where human landing rates are measured

6. Area 3: Treatment with traps 113m x 55m = 6215 sqm 20 m M M Biogents traps Ovitraps (for monitoring deposited eggs) Position, where human landing rates are measured

7. Area 4: Control 30 m M Ovitraps (for monitoring deposited eggs) Position, where human landing rates are measured

8. Area 5: Control 30 m M Ovitraps (for monitoring deposited eggs) Position, where human landing rates are measured

9. Area 6: Control 30 m M Ovitraps (for monitoring deposited eggs) Position, where human landing rates are measured

10. May June July August September October Mean human landings per hour With traps No traps Summary of all three areas: Reduction of human landing rates

11. Summary of all three areas: Reduction of egg production

12. 4950 759 Areas without traps 67,41612Total number of colleceted eggs in ovitraps 89,381Total human landing counts Reduction in %Areas with traps From 1 August to 30 September Summary