1. AFM Analysis of Gallid Herpesvirus 1
Casey Ross Mullikin
REU Faculty Mentor: Dr. Steve Tung
REU Graduate Mentor: Brock Schulte

2. Objective
Utilize Atomic Force Microscopy to measure electrical properties of Gallid Herpesvirus 1 (Chicken Herpes)
Use Viral electrical properties as a detection scheme and identification tool

3. AFM Schematic

4. Equipment
Scanner
Agilent 5500 AFM
Nose Cone

5. Cantilever Tip Type Used Dimensions Materials Contact Surf 0.2 N/m
13 kHz
Dimensions
Length: 450 μm
Width: 50 μm
Thickness: 2 μm
Materials Si
Cr/Pt

6. Herpesvirus Diagram

7. Virus Known as Properties Gallid Herpesvirus 1 Avian Herpesvirus 1
Infectious Laryngotracheitis
Chicken Herpes
Properties
Spherical nm
RNA Virus
5 Glycoprotein Subtypes

8. Scans
Beads
Virus

9. Impedance Impedance Analyzer Like resistance
Measurement of opposition to a sinusoidal current
Imaginary number in the form: Z=R+jX
Reactance is a dielectric property of the substance
If X>0 it is considered to be Inductive
If X<0 it is considered to be Capacitive
Absolute Impedance (Z) and Phase Angle (θ) were Measured
Impedance Analyzer

10. Impedance Values Frequency (Hz) ITO (Ω) Bead (Ω) Virus (Ω) Buffer (Ω)
%Difference
100000
5.37
150000
4.67
200000
3.30
250000
2.00
300000
8.76
350000
15.86
400000
26.91
450000
15.32
500000
0.81
550000
3.33
600000
1.15
650000
3.62
7.59

13. Conclusions
The overall Dielectric Constant of the virus was determined to be approximately 2.4
This value should be unique to this virus and thus allow for its identification via an infected sample analysis.

14. References
*1 Research Solutions & Resources LLC.
*2 Comparison of Electrical Properties of Viruses Studied by AC Capacitance Scanning Probe Microscopy.
Robert I. MacCuspie, Nurxat Nuraje, Sang-Yup Lee, Anne Runge, and Hiroshi Matsui
*3 ZView Download.
*4 Human Herpes Virus 3.
&

15. Questions?