1. AppliChem © 2010 1/14 There is another top address in Darmstadt! AppliChem CheLuminate

2. AppliChem © 2010 2/14 There is another top address in Darmstadt! Chemiluminescence detection kits replaces radioactive assays extremely sensitive  CheLuminate

3. AppliChem © 2010 3/14 There is another top address in Darmstadt! Horseradish Peroxidase-driven Luminol-based plus enhancer New: plus nucleophilic acylation catalyst  CheLuminate

4. AppliChem © 2010 4/14 There is another top address in Darmstadt! Chemiluminescence detection kits o for ELISA o for highest sensitivity o for standard applications (with phenolic enhancer)  CheLuminate-HRP ELISA FemtoDetect  CheLuminate-HRP ELISA FemtoDetect Plus  CheLuminate-HRP FemtoDetect  CheLuminate-HRP FemtoDetect Plus  CheLuminate-HRP PicoDetect Extended  CheLuminate-HRP PicoDetect  CheLuminate

5. AppliChem © 2010 5/14 There is another top address in Darmstadt! AppliChem's products: 10 -12 10 -14 10 -15  CheLuminate

6. AppliChem © 2010 6/14 There is another top address in Darmstadt! Sensitivity and stability Comparison of CheLuminate PicoDetect Extended with a competitor (B)  CheLuminate

7. AppliChem © 2010 7/14 There is another top address in Darmstadt! AppliChem CheLuminate – Sales arguments o competitive price o highest sensitivity available – from pico (10 -12 ) to femto (10 -15 ) range o extremely high stability of reagents – up to 24 h signal duration o compatible with most detection systems / instruments  CheLuminate

8. AppliChem © 2010 8/14 There is another top address in Darmstadt! Competitors o Pierce (Thermo Scientific) Pierce ECL, SuperSignal™ West Pico, SuperSignal™ West Femto o Millipore Immobilon™ Western HRP o GE healthcare ECL™  CheLuminate

9. AppliChem © 2010 9/14 There is another top address in Darmstadt! Literature  CheLuminate

10. AppliChem © 2010 10/14 There is another top address in Darmstadt!  Bio-/Chemiluminescence Luciferase System o Luciferase is one of the most sesnitive reporter in gene expression studies; no radioactivity o No cellular background activity of luciferase in most assay systems o Linear activity over a wide range o Easy to perform APMBT Zu bearbeiten

11. AppliChem © 2010 11/14 There is another top address in Darmstadt!  Bio-/Chemiluminescence Luciferase System with APMBT o Luciferase from Photinus pyralis (Pluc) o Luciferase from Renilla reniformis (Rluc) o application of both luciferases in the same assay possible: internal control or investigation of two transcription signals o This assay is compatible with protein assays!* o first, measurement of Pluc, then Rluc under Rluc assay conditions, Pluc inactive, because APMBT quenches Pluc Zu bearbeiten o APMBT = 2-(4-Aminophenyl)-6-methylbenzothiazole) o * Reference: Hampf, M. & Gossen, M. (2006) Anal. Biochem. 356, 94-99 o comparable to Promega‘s DualGlow

12. AppliChem © 2010 12/14 There is another top address in Darmstadt!  Bio-/Chemiluminescence BCA o Simple, sensitive photometric assay for proteins. o Faster and easier than Lowry assay. o and simple buffer salts. More stable at alkaline pH. o With greater tolerance to interference from non-ionic detergents, Zu bearbeiten o The principle of the bicinchoninic acid (BCA) assay is similar to the Lowry procedure, in that both rely on the formation of a Cu2+-protein complex under alkaline condition followed by reduction of the Cu2+ to Cu1+. o The amount of reduction is proportional to the protein present. In the second step, BCA forms a complex with Cu1+, which is purple colored and is detectable at 562 nm. o Protein + Cu2+ => Cu1+ + BCA => Cu1+-BCA complex o market leader probably Pierce

13. AppliChem © 2010 13/14 There is another top address in Darmstadt!  Bio-/Chemiluminescence e.g. Histology Alkaline Phosphatase Detection: BCIP / NBT Diformazan e.g. Cloning Blue/White Selection: X-Gal / IPTG e.g. Reporter Genes Firefly Luciferase Detection: Luciferin Light e.g. Western Blots Horseradish Peroxidase Detection: Luminol Light H2O2H2O2 ATP o substrates & inducers for the detection of genes & proteins, enzyme activities Overview CheLuminate-HRP Zu bearbeiten