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Chapter 8 Section 8.5: Translation 1
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Objectives SWBAT describe how mRNA codons are translated into amino acids. SWBAT summarize the process of protein synthesis. 2
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Vocabulary Translation Codon Stop codon Start codon Anticodon 3
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The Process of Translation Translation is the process that converts, or translates, an mRNA message into a polypeptide. – One or more polypeptide chains make up a protein. While DNA and RNA only use four nucleotides each in their codes, the language of proteins uses 20 amino acids. 4
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Triplet Code A codon is a three- nucleotide RNA sequence that codes for an amino acid. The genetic code matches each codon to its amino acid or function (the start/stop codons). 5
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Triplet Code Methionine is the start codon while there are three stop codons. 6
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Triplet Code – for Amino Acids A change in the order in which codons are read changes the resulting protein. Regardless of the organism, codons code for the same amino acid. 7
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Anticodon An anticodon is a set of three nucleotides that is complementary to an mRNA codon (a codon of CCC binds with an anticodon of GGG). An anticodon is carried by a tRNA. 8
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Ribosomes (rRNA) The large and small ribosomal subunits pull mRNA through the ribosome, reading it one codon at a time. The large subunit has three binding sites for tRNA’s anticodon. 9
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Translation 10 For translation to begin, tRNA binds to a start codon and signals the ribosome to assemble. A complementary tRNA molecule binds to the exposed codon, bringing its amino acid close to the first amino acid.
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Translation The ribosome helps form a polypeptide bond between the amino acids. The ribosome pulls the mRNA strand the length of one codon. 11
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Translation The now empty tRNA molecule exits the ribosome. – A complementary tRNA molecule binds to the next exposed codon. – Once the stop codon is reached, the ribosome releases the protein and disassembles. 12
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