1. LIVE ON CLONING ENTERPRISES

2. who is Live on?
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the goal here at live on is to research and find a new and more efficient way of cloning organisms. we believe that the discoveries and possibilities in the field of cloning are endless. the money that you would be investing is going straight toward the research aspect of our company, because cloning is not cheap

3. HISTORY
cloning is a term that means using the DNA from an organisms and creating an exact copy organism
this has actually been done since the beginning of time when it came to plants, as people began to take cuts from plants and plant them to where they would grow
Tissue culture came into commercial use in the 1950's, primarily to reproduce orchids, and in the 1970's its use with other plants became widespread.
the first animals to be cloned were frogs in the early 1950's

4. how it works

5. The break down 1. Isolate donor nucleus
Isolate the nucleus from a somatic (non-reproductive) cell of a adult donor sheep. The nucleus contains the complete genetic material of the organism. This step is repeated many times to gather many cell nuclei. A very small needle and syringe (suction device) is used to poke through the cell membrane to capture the nucleus and remove it from the cell.
2.Get unfertilized eggs
Retrieve unfertilized egg cells (reproductive) from a female sheep. Many eggs are needed since not all of them will survive the various steps of cloning.
3. Remove the egg's nucleus
Remove the egg cell's nucleus, which contains only one-half of the sheep's genetic material. A very small needle and syringe (suction device) is used to poke through the cell membrane to capture the nucleus and remove it from the cell.
4. Insert donor nucleus
Insert the nucleus, with its complete genetic material, isolated from the donor mammal in Step 1 into the egg cell that has no nuclear material. The egg's genetic material now contains all traits from the donor adult. This egg is genetically identical to the donor adult.
5. Place the egg into womb
Place the egg into a female sheep's womb. Only a small percentage of eggs placed in the womb will start to mature. Those eggs that survive will continue to develop into embryos. The egg matures in the womb. When the offspring is born, it is a clone (genetically identical) of the donor sheep.

6. current applications Food livestock
in this very day and age, cloning can be used to clone mammals such as prize bulls or race horses. it is very expensive which is the reason it isn't being done to an extent. although it is being studied extensively it isn't being done as much as some would hope because of the price, and the humane aspect of it
Food
livestock
another application for cloning now is for a food source. we are using this now to create more and more animals to eat in our everyday lives. the issue with this is that the fda, although states its ok, will not stamp aproval on any cloned meat

7. conservation
one of the other main points of mammal cloning would be the conservation view point. right now the endangered species list grows more and more each day. cloning is a way to take the endangered species list down completely. if we could clone every animal there that is struggling enough to where they can be put back into the wild then the right thing has been done

8. low efficiency= high death
BIOETHICS
when it comes to cloning animals there are definitely some ethical implications. the humane aspect of it is what gets to people, to proceed with this process you have to take out and fertilize over 300 cells and maybe only one will actually begin to grow. that one that may grown has a very low survival rate due to the factors that come into affect with cloning.
low efficiency= high death
laws
when it comes to laws, the FDA has approved that cloned meat is no different than normal, so cloning in the aspect of livestock is legal. as is the field of cloning pets, but the hassle and price of cloning an animal is just too much to handle

9. BIOETHICS WHY WE ARE DIFFERENT
our organization is going to use your funds to actually study new ways to go about cloning without having to break ethical code. once we find a better way to do so, we can work our way up to small animals, to pets, to livestock and large animals. this is a necessary process because the new efficient way of cloning should be perfected before starting on the next size animal. this way we are not using so many fertilized cells and throwing them away because they don't grow.
on the human side of cloning there many laws in the United States and other countries that pertain to human cloning. the laws that are country wide pertain to the actual cloning of humans; it is highly illegal. but the research end of the spectrum is very legal. scientists are not at all allowed to attempt a human clone but can research in alternative ways. at live on we don't believe that human cloning should be allowed or ever attempted so we will not be going into any detail on that aspect of cloning.q
WHY WE ARE DIFFERENT

10. PROS AND CONS OF CLONING
reproduction of food supply animals
cloned animals have 1/2 the lifespan of donor
perfection- cloning could take out all informalities and generally create the perfect breed
inhumane- the thought of many is that it is unhumane
endangered species- could erase the entire endangered species list
not stamped- the FDA will not approve any cloned meat which cause controversy
discoveries- there is no telling to what didcoveries about the world or the human race could be discovered through cloning research

11. Dolly
The first larger animal to be cloned by using the nuclear approach was a sheep in Switzerland in it was the first mammal to be cloned successful.
the idea to clone a sheep was introduced by a farmer in Switzerland and it actually worked. the scientists actually went through the process of taking the cells from the mammary gland of the donor sheep, putting that exact nucleus with the dna inside the egg cell of a surrogate mother, and placed it back into the surrogate and then dolly was born