1. With all thine offerings thou shalt offer salt - Moses (in Leviticus)

3. Why salt?

4. Biological Need For Salt

5. Salt Sources

6. Gluphisia septentrionis

7. Puddling

8. Why?

9. Gluphisia, Salt & Reproduction

11. Sampling Protocol Using dropper pipette, extract 1mL from petri dish Place 1mL sample into petri dish lid Place lid and sample on top of graph paper and view under stereoscope Count hatched naupili Multiply total number of naupili from sample by 30 Repeat 3x for each culture/petri dish Average results for each culture and post results. Use class averages to support/refute conclusions Clean out dishes, remove labels, place in wash bin (center island)

12. Lab Report Write in lab notebook Title Purpose w/general methodology Hypothesis w/rationale (reasoning) Data table (class avg), graph and qualitative observations Conclusion –Restate hypothesis –Supported, refuted or inconclusive –Selected data citations and elaboration –What does this suggest about the relationship of the independent variable and dependent? –Were there any sources of error/confounding variables and how might they have influenced your conclusion?