1. South/Central America Regional Meeting, Campinas, Brazil, 19 March 2007 CBOL Working Groups David E. Schindel, Executive Secretary National Museum of Natural History Smithsonian Institution SchindelD@si.eduSchindelD@si.edu; http://www.barcoding.si.edu http://www.barcoding.si.edu SchindelD@si.eduhttp://www.barcoding.si.edu 202/633-0812; fax 202/633-2938

2. South/Central America Regional Meeting, Campinas, Brazil, 19 March 2007 CBOL’s Working Groups Database: Designing/constructing the BARCODE data standards Database: Designing/constructing the BARCODE data standards DNA: Protocols for formalin-fixed and old museum specimens; Producing LIMS for dissemination DNA: Protocols for formalin-fixed and old museum specimens; Producing LIMS for dissemination Data Analysis: Beyond phenetic methods; population genetics perspective Data Analysis: Beyond phenetic methods; population genetics perspective Plants: Identify gene region(s) for barcoding Plants: Identify gene region(s) for barcoding

3. South/Central America Regional Meeting, Campinas, Brazil, 19 March 2007 BARCODE Data Standards Consensus results of Front Royal meeting Consensus results of Front Royal meeting GBIF ITIS GRINGBIF ITIS GRIN NBII Species2000 IPNINBII Species2000 IPNI ICZN ZooRecord OBISICZN ZooRecord OBIS Structured link to voucher specimen Structured link to voucher specimen Species name selected from authority Species name selected from authority Online access to metadata Online access to metadata Trace files and quality scores Trace files and quality scores Minimum sequence length Minimum sequence length

4. South/Central America Regional Meeting, Campinas, Brazil, 19 March 2007 Barcode Sequence Voucher Specimen Species Name Specimen Metadata Literature (link to content or citation) BARCODE Records in INSDC Indices - Catalog of Life - GBIF/ECAT Nomenclators - Zoo Record - IPNI - NameBank Publication links - New species Databases - Provisional sp. Georeference Habitat Character sets Images Behavior Other genes Trace files Other Databases Phylogenetic Pop’n Genetics Ecological Primers

5. South/Central America Regional Meeting, Campinas, Brazil, 19 March 2007 Digitizing Taxonomic Literature CBOL’s catalytic efforts: CBOL’s catalytic efforts: Library-Laboratory meeting in London, 2005, on electronic access to taxonomic literatureLibrary-Laboratory meeting in London, 2005, on electronic access to taxonomic literature Led to formation of Biodiversity Heritage Library initiativeLed to formation of Biodiversity Heritage Library initiative Industrial-scale digitization of pre-1923 taxonomic literature; free online accessIndustrial-scale digitization of pre-1923 taxonomic literature; free online access OCR and Taxonomic IntelligenceOCR and Taxonomic Intelligence Partner in Encyclopedia of Life informaticsPartner in Encyclopedia of Life informatics

6. South/Central America Regional Meeting, Campinas, Brazil, 19 March 2007 CBOL’s Working Groups Database: Designing/constructing the BARCODE data standards Database: Designing/constructing the BARCODE data standards DNA: Protocols for formalin-fixed and old museum specimens; Producing LIMS for dissemination DNA: Protocols for formalin-fixed and old museum specimens; Producing LIMS for dissemination Data Analysis: Beyond phenetic methods; population genetics perspective Data Analysis: Beyond phenetic methods; population genetics perspective Plants: Identify gene region(s) for barcoding Plants: Identify gene region(s) for barcoding

7. South/Central America Regional Meeting, Campinas, Brazil, 19 March 2007 CBOL Formalin Workshop May 8-9, 2006 workshop in Washington, National Research Council May 8-9, 2006 workshop in Washington, National Research Council Literature survey of DNA recovery protocols from formalin-fixed specimens Chemists, biochemists, biophysicists, biomedical researchers Literature survey of DNA recovery protocols from formalin-fixed specimens Chemists, biochemists, biophysicists, biomedical researchers Create a new research agenda Create a new research agenda Follow-on with SPNHC as major partner Follow-on with SPNHC as major partner

8. South/Central America Regional Meeting, Campinas, Brazil, 19 March 2007 CBOL’s Working Groups Database: Designing/constructing the BARCODE data standards Database: Designing/constructing the BARCODE data standards DNA: Protocols for formalin-fixed and old museum specimens; Producing LIMS for dissemination DNA: Protocols for formalin-fixed and old museum specimens; Producing LIMS for dissemination Data Analysis: Beyond phenetic methods; population genetics perspective Data Analysis: Beyond phenetic methods; population genetics perspective Plants: Identify gene region(s) for barcoding Plants: Identify gene region(s) for barcoding

9. South/Central America Regional Meeting, Campinas, Brazil, 19 March 2007 CBOL’s Working Groups Database: Designing/constructing the BARCODE data standards Database: Designing/constructing the BARCODE data standards DNA: Protocols for formalin-fixed and old museum specimens; Producing LIMS for dissemination DNA: Protocols for formalin-fixed and old museum specimens; Producing LIMS for dissemination Data Analysis: Beyond phenetic methods; population genetics perspective Data Analysis: Beyond phenetic methods; population genetics perspective Plants: Identify gene region(s) for barcoding by December 2006 Plants: Identify gene region(s) for barcoding by December 2006

10. South/Central America Regional Meeting, Campinas, Brazil, 19 March 2007 Progress toward Plant Barcode Kress 2005 proposal for ITS and trnh- psbA Kress 2005 proposal for ITS and trnh- psbA Kew Garden receives Sloan/Moore Foundation support, early 2005 Kew Garden receives Sloan/Moore Foundation support, early 2005 Phase 1 screens 100 genes across 50 sibling species pairs Phase 1 screens 100 genes across 50 sibling species pairs Phase 2 tests of matK, rpcoC1, rpoB, ndhJ, and accD Phase 2 tests of matK, rpcoC1, rpoB, ndhJ, and accD Canadian proposal for rbcL Canadian proposal for rbcL CBOL protocols for approving barcode regions CBOL protocols for approving barcode regions

11. South/Central America Regional Meeting, Campinas, Brazil, 19 March 2007 Wider Impacts of Barcoding Catalyzing interoperability of databases Catalyzing interoperability of databases Barcode data standards link sequences, specimens, species names and publicationsBarcode data standards link sequences, specimens, species names and publications Improving information infrastructure Improving information infrastructure Digital library initiative in taxonomyDigital library initiative in taxonomy Expanding analytical toolbox for taxonomy Expanding analytical toolbox for taxonomy Renewing the mission of museums Renewing the mission of museums DNA recovery from formalin-fixed specimensDNA recovery from formalin-fixed specimens