1. Circulating Cell-Free DNA Necrosis, apoptosis, and possibly “active secretion” are thought to be the mechanisms by which cell-free nucleic acids enter the circulation. 0.18Kb Amos Douvdevani, PhD Department of Clinical Biochemistry and Pharmacology, Soroka Medical Center & Ben-Gurion University of the Negev

2. Dilution of sera in buffer containing SYBR Gold stain Measurement of fluorescence Direct fluorometric Method for DNA Measurement Goldshtein et al. Ann Clin Biochem 2009; 46: 488–494 Mix & Measure, immediate quantitative results Cost of reagents $5/100 samples Circulating free DNA Could be tested in the clinic using a small device (DNAmeter) SYBR Gold stain Serum/ plasma 20  l

3. Few ideas for Possible collaboration Diagnosis of pneumonia (reduce X-ray) Diagnosis of flue and other viral diseases Early cancer detection Any idea you have (free assay for all) Amos Douvdevani, amosd@bgu.ac.il, Tel: 054-4403214amosd@bgu.ac.il