1. Arizona Molecular Diagnostic & Research Laboratories Robotics 2006

2. Shared Needs

3. Mission To provide clinically relevant molecular laboratory services for our patients, providers, and research staff through an integration of laboratory practice, research, and education. Service: Education: Research:

4. Joint Venture A collaboration of clinical and anatomic laboratories, designed to support molecular diagnosis of disease, provide education, and perform research Clinical Laboratory Directors Ronald Schifman, M.D.Ronald Weinstein, M.D. Donna Wolk, Ph.D.Anil Prasad, M.D. Margaret Rennels, M.D. Lisa Rimsza, M.D. Monika Schmelz, Ph.D.Christopher Cuniff, M.D.

5. AMDRL Translational Services Core Services, GLP  Nucleic Acid Extraction  Real-time PCR: QPCR and SNP  SNP Analysis with Invader Assays  Fluorescent Microscopy with Digital Imaging/Fluorescent In-Situ Hybridization  Molecular Epidemiology, rep-PCR  Confocal Laser Scanning Microscopy  Immunohistochemistry (digital scanning)  Biorepository Services  Misc. Clinical Laboratory Testing

6. GLP Research Core

7. MagNA Pure LC DNA Extraction Boom extraction technology adaption Lysis in chaotropic salts and proteinase K DNA bound to MGP’s, washed, & eluted 32 samples / 1-2 hrs.

8. Real-time PCR: ABI Sequence Detection System, 7900 HT, Taq 48 for viral loads

9. Molecular Epidemiology: Rep-PCR Also T-cell clonality

10. Invader Platform: SNP FVL, FII, HCV genotype

11. Microscopy & DNA Probes for Cancer (FISH) Her-2-Neu positive

12. Confocal Laser Scanning Microscopy Focal Contacts: Vinculin Actin (Phalloidin-FITC)

13. Opportunity: MAVERIC National VA Biorepository Processing, Storage, and Analysis Consent Process Technical Infrastructure Space and Infrastructure at VA Software System, TissueBase

14. Current Status  Providing infrastructure so that the latest technology can be acquired and used for diagnosis and clinical research purposes  Performing funded clinical research  Bioterrorism, Waterborne pathogens  Sepsis  Valley Fever  Community Acquired Pneumonia  Drug-resistant pathogens  Prostate Cancer and Bladder Cancer  GI Cancer  Skin Cancer

15. Current Status  Clinical Lab assays  Mycobacterium sp. By DNA probe  Coccidioides by DNA probe and real-time PCR  HCV real-time PCR  HIV RT-PCR  Molecular epidemiology, ORSA, VRE, others  CT/NG  Microsporidia by real-time PCR  Factor V Leiden  Prothrombin gene (Factor II)  FISH for bladder cancer  FISH for Her-2-neu

16. Thank you