1. Inhalation System for Animal Infection With BSL3 Pathogens Team Members: Stacey Hoebel Maja Middleton

2. Alternative Design: One-Compartment Box Modification of current lab design Modification of current lab design –Safer and more efficient One large compartment made of plastic able to withstand autoclave One large compartment made of plastic able to withstand autoclave –No need for individual compartments for animals –Small enough to fit under fume hood

3. Alternative Design: One-Compartment Box Tube from nebulizer into compartment to administer pathogen Tube from nebulizer into compartment to administer pathogen Tube from compartment to ethanol trap and HEPA filter Tube from compartment to ethanol trap and HEPA filter

4. Alternative Design: One-Compartment Box Pros Very inexpensive Very inexpensive Easy to manufacture Easy to manufacture Better quality Better quality –Plastic compartment –Seal around tubing and lid Cons Too simple Too simple Doesn’t address all problems Doesn’t address all problems –Contaminates entire body of animal –Inconsistent amount of infection administered to animals

5. Alternative Design: Central Porous Tubing Plastic box with lid Plastic box with lid –Split into six built-in compartments which hold one animal each Central Porous Tubing Central Porous Tubing –One tube runs from nebulizer then down middle of box, between compartments –Infectious gas expelled out of holes in tubing into individual chambers –Remaining gas led to HEPA filter and ethanol trap Restraint Device Restraint Device –Holds animal in place while being infected

6. Alternative Design: Central Porous Tubing

7. Pros Individual compartments and restraints keep animals separated, calm Individual compartments and restraints keep animals separated, calm Better control of agent Better control of agent Relatively inexpensive Relatively inexpensive Easy to manufacture Easy to manufactureCons Not accurate enough Not accurate enough –Impossible to regulate how much agent is administered to each animal

8. Final Design: Multi-Chamber w/ Nose Piece Plastic box with lid Plastic box with lid –Made of polymethyl pentene (autoclavable) –Split into four built- in compartments Moveable back wall to adjust to animals of various sizes Moveable back wall to adjust to animals of various sizes –Small enough to fit under fume hood

9. Final Design: Multi-Chamber w/ Nose Piece Split Tubing Split Tubing –Nebulizer leads into one tube –Router splits this tube into four separate tubes –Each tube leads to a different compartment –Another router condenses tubing into one tube leading to ethanol trap and HEPA filter

10. Final Design: Multi-Chamber w/ Nose Piece Neck Seal Neck Seal –“Wall” of stretchy material set 1.5 inches from front of compartment –Small hole in center of material for head and neck –Holds animal in place –Creates seal between head and body

11. Final Design: Multi-Chamber w/ Nose Piece Nose piece Nose piece –Cone formed at end of tubing by snipping each side and caulking between cut –Does not need to be directly attached to head –Directs airflow to nose and mouth

12. Final Design: Multi-Chamber w/ Nose Piece Pros Separate tube for each chamber Separate tube for each chamber –Consistent, efficient administration of pathogen Minimization of body contamination Minimization of body contamination –Nose piece directs airflow –Seal between head and body Able to sanitize entire system Able to sanitize entire system Many safeguards to prevent leakage of agent Many safeguards to prevent leakage of agent

13. Final Design: Multi-Chamber w/ Nose Piece Future Work Consult client about possibly anesthetizing animals Consult client about possibly anesthetizing animals –Complicated to monitor sedation levels –Could lose some animal subjects Organization system for all the tubing Organization system for all the tubing Appropriate materials (able to sterilize, non-porous) Appropriate materials (able to sterilize, non-porous)

14. Questions?