1. ASCOS Planned Aerosol Instrumentation Aboard Oden Douglas Orsini Jost Heintzenberg Leibniz Institute for Tropospheric Research Leipzig, Germany

2. Motivation To contribute to measured physical and chemical properties of the marine aerosol ( especially Dp < 1 um) and to target biological particles Three instruments: 1.DMPS 1.VTDMA 1.Water Cyclone

3. DMPS Differential Mobility Particle Sizer Goal: To measure the aerosol size distribution Particle size range: 3 nm < Dp < 800 nm Sample Flow Rate:1 lpm Measurement Rate:Semi-continuous every 10 min.

4. VTDMA Volatility Tandem Differential Mobility Particle Analyzer Goal: To infer composition and mixing state based on reference volatility measurements 1. Select particle size (30 nm < Dp < 300 nm; 1 lpm) 2. Heat the selected aerosol size ( 3 temperatures possible ) 3. Re-measure the aerosol size

5. VTDMA Volatility Tandem Differential Mobility Particle Sizer Some reference temperatures: ~ 150 - 200 o C ( H 2 SO 4, (NH 4 )HSO 4, (NH 4 ) 2 SO 4 ) ~ 300 o C ( volatile OC ) ~ 600 - 800 o C ( less volatile OC + NaCl ) ~ ? ( viruses, bacteria, cell material ) - fine marine aerosols exist capable of surviving at 300 o C (Clarke, 1999, Clarke et al., 2003) - little data on volatility of viruses and bacteria as ambient particles To Be Determined: selected sizes + heating temperatures Time permitting: prelimin. tests of bubbled microlayer water to determine temperatures.

6. Water Cyclone Collects bulk aerosol into liquid (insoluble + soluble) Sample flow rate:17 lpm continuous Liquid sample flow: 24 hours: 100 - 1000 ml of sample liquid for analysis Consumables: 0.02  ultrapure water : 5 liters / day

7. Liquid Sample Analysis Water Cyclone On-Board Biological Enumeration Storage for Post Analysis Chemical and Biological

8. Water Cyclone On-Board Biological Enumeration Ship-board analysis of aerosol sample: (pending fluorescence microscope) 1. Concentrate sample liquid on.02  filter substrate (start with 24 hr. sample) 2. Stain with Sybr-Green/Gold which binds to nucleic acid 3. Enumerate biological particles (based on known protocol) with microscope

9. Post Analysis Water Cyclone microlayer liquid & bubbled microlayer aerosol collection into liquid Coordinate similar sample preparation and storage protocols e.g.: TOC Basic ions Amino acids EPS gels flow cytometry..

10. What is possible for consistent analysis ? microlayer liquid & bubbled microlayer aerosol collection into liquid fogwater aerosol impactors Coordinate parallel sample storage and protocols

11. An upper Dp size cut-off ? e.g. : Water Cyclone PM10 aerosol impactor Dp < 100 nm collection into liquid

12. Counting Protocol for Marine Samples