1. Experiment 6 Cultivation and Identification of Virus

2. Culture of virus Culture in experimental animal Culture in chicken embryo Culture in cell: *primary cultural cells *diploid cell strain *continuous or infinite cell line of strain

3. Culture in experimental animal

4. Culture in chicken embryo

5. 气室 卵壳 壳膜 尿囊腔 绒毛尿囊膜 胚胎 羊膜腔 卵黄 卵白

6. 天花病毒， 痘病毒和 HSV 流感病毒， 腮腺炎病毒 流感病毒 初次分离 嗜神经病毒

7. Culture in cell: *primary cultural cells *diploid cell strain *continuous or infinite cell line of strain

8. 动物细胞原代培 养过程图

9. *Identification in cell culture ： 1) Cytopathic effect (CPE ) : 2) Inclusion body ： 3 ） Hemadsorption ： 4 ） Interference with formation of a CPE by a second virus ：

10. 细胞病变效应CPE 正常细胞

11. Negri body in a neuron

12. Hemagglutination test and Hemagglutination inhibition test 1.Hemagglutination test hemagglutinin HA ： the glycoprotein spike encoded by the viral genes.Adsorption and enter into the host cells with the virus. HA can bind with N-acetyl neuraminic acid enzyme receptor from erythrocyte of human or other animals and cause hemagglutination.

13. Principles of Hemagglutination inhibition test hemagglutinin Phenomenon caused by virus can be inhibited by corresponding anticoagulant antibody,this is called hemagglutination inhibition. hemagglutinin specific antibodies interact with corresponding HA of virus surface,so the phenomenon that inhibit virus hemagglutinin is called hemagglutinin inhibition test.

14. 实验课考试： 考试内容－细菌的单染 注意事项－ 无菌操作、制片、染色、显微镜使用 请每人准备一张空白纸，在左上角写上本人 姓名和学号。