1. The Prokaryotes Chapter 16

2. Virus Bacterium Animal cell Animal cell nucleus 0.25 µm

3. Unicellular Prokaryotic cells have no nucleus –DNA is a single, circular chromosome DNA is in an unbound region called the nucleoid –Prokaryotic cells lack membrane-bound organelles Many bacteria also have plasmids –smaller circular DNA molecules that can replicate independently of the chromosome Bacterial cells divide by binary fission Genetic variation mainly by mutations –Since bacteria can reproduce rapidly, new mutations quickly increase genetic diversity Cell Wall surrounds plasma membrane Comparing Prokaryotic and Eukaryotic Cells

4. Bacterial Genome and Its Replication The bacterial chromosome is usually a circular DNA molecule with few associated proteins Many bacteria also have plasmids, smaller circular DNA molecules that can replicate independently of the chromosome Bacterial cells divide by binary fission Origin of replication Replication fork Termination of replication

5. A typical rod-shaped bacterium A thin section through the bacterium Bacillus coagulans (TEM) 0.5 µm Pili Nucleoid Ribosomes Plasma membrane Cell wall Capsule Flagella Bacterial chromosome

6. Prokaryotic Classification Domain Bacteria vs Archaea Cell Wall composition –Gram negative or gram positive Cell shape Mode of nutrition Molecular characteristics –rRNA sequence comparisons

7. Composition of Cell Wall: Gram-positive vs Gram-negative bacteria Cell Wall surrounds plasma membrane –Composed of lipids, carbohydrates and protein Peptidoglycan (mix of protein and carbohydrates) –NO Cellulose (plants cells) –Provides structure and support Structure of cell wall categorize bacteria based on ability to retain the Gram stain Series of two staining procedures –Crystal violet + iodine –Ethanol rinse –Safranin counterstain

8. Gram-Positive Extra thick peptidoglycan layer allows cells to RETAIN primary stain and thus appear BLUE Bacillus anthracis

9. Gram Negative Do NOT retain primary stain and thus take in the PINK counterstain Most PATHOGENIC bacteria are Gram-Negative!!

10. Some Prokaryotes also may exhibit: Capsules –a sticky layer of polysaccharides or protein. shields pathogenic prokaryotes from attacks by a host’s immune system. Fimbriae –Hairlike projections enable prokaryotes to stick to their substrate or each other Flagella –help prokaryotes move in their environment Produce endospores –Allow bacteria to remain dormant during harsh conditions Additional Features of Prokaryotic Cells Endospore Flagella Fimbriae Capsule Tonsil cell Bacterium

11. Roles of Bacteria in Ecosystems and Human Health Ecosystems –Decomposers Recycle nutrients that would otherwise remain unavailable in dead organisms and waste –Producers Important primary producers in oceans and lakes Human Health –Source of antibiotics –Aid in digestion and vitamin synthesis Disease Fermented foods –Yogurt –Cheese –Soy sauce

12.  All organisms are almost constantly exposed to pathogenic bacteria.  Most bacteria that cause illness do so by producing a poison. Exotoxins are proteins that bacterial cells secrete into their environment. Endotoxins are components of the outer membrane of gram-negative bacteria. Some bacteria cause disease © 2012 Pearson Education, Inc. Exotoxin Secreted by cell Staphylococcus aureusSalmonella enteritidis Endotoxin Component of gram- negative plasma membrane

13. 1. find the bacterium in every case of the disease, 2. isolate the bacterium from a person who has the disease and grow it in pure culture, 3. show that the cultured bacterium causes the disease when transferred to a healthy subject, and 4. isolate the bacterium from the experimentally infected subject. Koch’s postulates are used to prove that a bacterium causes a disease © 2012 Pearson Education, Inc.

14. Mutation and Genetic Recombination as Sources of Genetic Variation Rapid reproduction, mutation, and genetic recombination contribute to the genetic diversity of bacteria Since bacteria can reproduce rapidly, new mutations quickly increase genetic diversity Additional genetic diversity arises by recombination of DNA from two different bacterial cells Three processes bring bacterial DNA from different individuals together: –Transformation –Transduction –Conjugation

15. Bacteria can transfer DNA in three ways  Bacteria use three mechanisms to move genes from cell to cell. 1.Transformation is the uptake of DNA from the surrounding environment. 2.Transduction is gene transfer by phages. 3.Conjugation is the transfer of DNA from a donor to a recipient bacterial cell through a cytoplasmic (mating) bridge.  Once new DNA gets into a bacterial cell, part of it may then integrate into the recipient’s chromosome. © 2012 Pearson Education, Inc.

16. A+A+ Phage DNA A+A+ Donor cell B+B+ A+A+ B+B+ Crossing over A+A+ A–A– B–B– Recipient cell A+A+ B–B– Recombinant cell Sex pilus F plasmidBacterial chromosome F + cell Mating bridge F + cell Bacterial chromosome F – cell Conjunction and transfer of an F plasmid from and F + donor to an F – recipient http://highered.mheducation.com/sites/dl/free/0072835125/126997/animation6.html

17. Antibiotics Target Unique Features of Prokaryotes 3 major target of antibiotics: –Enzymes required for cell wall synthesis –Bacterial ribosomes (interfere with protein synthesis) –DNA replication in bacteria All of these drugs inhibit or slow bacterial growth by inhibiting enzymes or cellular structures unique to bacteria Not effective against viruses Bacterial cells in a population resistant to antibiotic will continue to grow, selecting for antibiotic resistant strains http://www.sumanasinc.com/scienceinfocus/antibiotics/antibiotics_fla.html

18. Evolution Connection: The Evolution of Antibiotic Resistance –Many antibiotics disrupt cellular structures of invading microorganisms. –Introduced in the 1940s, penicillin worked well against such infections. –But over time, bacteria that were resistant to antibiotics, such as the MRSA strain, were favored. –The widespread use and abuse of antibiotics continue to favor bacteria that resist antibiotics. © 2013 Pearson Education, Inc.

19. Figure 4.23