1. WP6 presentation Quality of oats : protein and oil contents Micronutriments and avenins AVEQ meeting BUCHAREST 19-21/10/2010

2. Partners for this workpackage : AVEQ meeting BUCHAREST 19-21/10/2010 France : Jean Koenig INRA Clermont-Ferrand Gérard Branlard INRA Clermont-Ferrand Audrey Didier INRA Clermont-Ferrand External service : Vetagro sup Agnès Piquet Estonia : Külli Annamaa Jogeva Plant Breeding Institute Romania : Danela Murariu Genebank Suceava Manuela Ibanescu Genebank Suceava

3. Preliminary work to analyses : Dehulling of seeds Done by Alf Seplitis (Partner 2) and Mathias Hermann (Partner 3) Hand dehulling has and will be done by IHAR Radzikov and PBI (Estonia) by hand which is a quite long work Thanks to them Milling has been done by laboratories carrying out NIRS analyses (problems of sticky flour..... ) AVEQ meeting BUCHAREST 19-21/10/2010

4. Near Infrared Spectra analysis (NIRS) On samples 2008, have been realized for 6 locations : BUL, EST, FRA, ITA, ROM, POL, and on samples 2009 are on the way. Calibration by Kjeldahl (proteins) and (Fat) and Calibration verification have been done on 2008 samples. Calibration values will be entered in the NIRS software to produce protein and fat values.

5. Protein content F irst results from calibration by Kjeldahl method show a wide range of variation : within locations and looking at all locations AVEQ meeting BUCHAREST 19-21/10/2010

6. Micronutriments Have been realized on 122 (100 selected by PCA + 2 X 11 Standards) samples from 2008 (mineral micronutriments)on samples from french harvest. But results were not produced yet by partner (external) Samples have been chosen on spectra from NIRS analysis (PCA) choosing more diverse accessions Will be done on samples from France 2009 in the next months AVEQ meeting BUCHAREST 19-21/10/2010

7. Results for avenins patterns

8. Avenin identification Avenin extraction Allele identification for the 3 loci: Ave1, Ave2 et Ave3 1D electrophoresis Acide-Page Method

9. Identification of alleles of Ave1 locus 5 alleles

10. Identification of alleles of Ave2 locus 6 alleles

11. Identification of alleles of Ave3 locus 10 alleles

12. 22.4 % of accessions were heterogenous Genetic heterogeneity within accession Grain mixture within accession Homogeneity (purety) of the Avena sativa patterns as revealed through avenin analysis 80% old accessions 20% modern accessions

13. Locus identifiéEffectif Pourcentage (N=543) Ave130 5.5 Ave222 4.0 Ave353 9.8 Ave1 + Ave229 5.4 Ave1 + Ave2 + Ave315228.0 Ave1 + Ave376 14.0 Ave2 + Ave399 18.2 Aucun82 15.1 Identification of avenin alleles in A. sativa

14. Alleles repartition at locus Ave1 ( N=300, 2008)

15. Alleles repartition at locus Ave2 ( N=300, 2008)

16. Alleles repartition at locus Ave3 ( N=300, 2008)

17. Thanks for your attention

18. AVEQ meeting BUCHAREST 19-21/10/2010 Overview of deliverables WP6