1. Christoph U. Germeier, Zofia Bulinska-Radomska, Lena Dimberg, Matthias H. Herrmann, Jean Koenig, Gerard Branlard, Danela Murariu, Ivana Polisenska, Rita Redaelli, Valeria Terzi Avena Genetic Resources for Quality in Human Consumption (AVEQ)

2.  Project Partners Owies, Kraków 2009 AVEQ - Outline  Objectives  Work plan  Fusarium pathogens and mycotoxins in oats  Introduction

3. A European Project on Nutritional Quality in Avena Genetic Resources Owies, Kraków 2009 AVEQ - Introduction Targeted action in accordance with the Council regulation (EC) N o 870/2004 establishing a Community programme on the conservation, characterisation, collection and utilisation of genetic resources in agriculture Starting date: 01. 03. 2007 End date: 28. 02. 2011 Duration: 48 months

4. Owies, Kraków 2009 AVEQ - Objectives Evaluation, characterisation and documentation of selected oat genetic resources with regard to:  Contents of protein, fat, minerals, starch, ß- glucan and antioxidants  Resistance to Fusarium infection and mycotoxin contamination  Cold tolerance  Grain weight, hull content and test weight

5. Owies, Kraków 2009 AVEQ:15 Partners from 9 countries Co-ordinator P0:Julius Kuehn Institute, Federal Research Centre for Cultivated Plants - Institute for Breeding Research on Agricultural Crops, Quedlinburg, Germany Project leader – Dr. Christoph U. Germeier P1: Plant Breeding and Acclimatization Institute (IHAR), National Plant Genetic Resources Centre Radzików, Poland P2: Svalöf Weibull AB Cereal Breeding Department, Svalöf, Sweden P3: Julius Kuehn Institute, Federal Research Centre for Cultivated Plants - Institute for Breeding Research on Agricultural Crops, Groß Lüsewitz, Germany P4: Eurofins Analytik GmbH, Hamburg, Germany

6. Owies, Kraków 2009 AVEQ - Partners P 9: Vegetal Genetic Resources Bank of Suceava, Suceava, Romania P8: CRA-GPG, ex Istituto Sperimentale per la Cerealicoltura, Fiorenzuola d'Arda, Italy P5: Institut National de la Recherche Agronomique (INRA), UMR Amélioration et Santé des Plantes, Clermont-Ferrand, France P6: CRA-MAC, ex Istituto Sperimentale Cerealicoltura, Bergamo, Italy P7: Swedish University of Agricultural Sciences, Department of Food Science, Uppsala, Sweden

7. Owies, Kraków 2009 AVEQ - Partners P11:Jõgeva Plant Breeding Institute, Jögeva, Estonia P10: Institute of Plant Genetic Resources “K. Malkov”, Sadovo, Bulgaria P12:Plant Breeding and Acclimatization Institute - Laboratory of Nutritional Evaluation of Plant Materials, Radzików, Poland P14: Agricultural Research Institute Kromeriz, Ltd., Czech Republic P15: Agrotest Fyto, Ltd., Kromeriz, Czech Republic

8. Owies, Kraków 2009 AVEQ - Materials Working collection of 600 accessions of Avena genetic resources of different improvement status :  accessions of wild species  selected land races  modern cultivars

9. Owies, Kraków 2009 Methods – Workpackages (WP)  WP 1: Management and Coordination - Julius Kuehn Institute, Institute for Breeding Research on Agricultural Crops, Quedlinburg, Germany, Christoph U. Germeier (P0)  WP 2: Project working collection - IHAR, Poland, Zofia Bulinska-Radomska (P1); P0, P5, P6, P8, P9, P10 Plan: 80% cultivated material - 200 landraces, 200 obsolete cultivars, 100 modern cultivars 20% wild Avena species (100 accessions).

10. WP 2: Project working collection Species20082009 A. sativa287256 A. byzantina816 A. strigosa1531 A. abyssinica23 A. barbata31 A. canariensis-1 A. damascena2- A. fatua79 A. hirtula13 A. hybrida1- A. sterilis23 A. wiestii1- 329323 Wild species not obtained A. agadiriana7 A. atlantica10 A. insularis1 A. longiglumis16 A. macrostachya2 A. murphyi16 A. occidentalis13 A. pilosa9 A. prostrata13 A. vaviloviana12 A. ventricosa8

11. 11 WP 2: Origin of the Project Working Collection

12. Owies, Kraków 2009 Methods - Workpackages WP 3: Field experiments and sampling seeds for quality analysis - Vegetal Genetic Resources Bank of Suceava, Romania, Danela Murariu (P9); P1, P2, P5, P8, P10, P11  Field experiments laid out as augmented block designs  Plot size from 2.0m 2 to 3.0m 2  establishing plots, taking observations in the field and on harvested material - descriptors

13. 13 IHAR SVGB IPGR INRA SW CRA-ISC PBI Descriptors observed in field experiments -Days to heading -Days to maturity -Plant height - Lodging - Panicle shape - Occurrence of diseases - Colour of lemma - Hull content - Yield - Thousand kernel weight - Test weight Obligatory:Optional: -Emergence -Number of fertile tillers -Number of seeds per panicle Owies, Kraków 2009

14. Methods – Workpackages WP 4: Field experiments with artificial Fusarium inoculation - Julius Kuehn Institute, Institute for Breeding Research on Agricultural Crops, Groß Lüsewitz, Germany, Matthias H. Herrmann (P3); P8 (Italy), P9 (Romania), P14 (Czech Republic)  Field trials, inoculation with Fusarium at 3 locations, in Italy natural infection  Observations in the field and on harvested material  Samples for mycotoxin analysis

15. Owies, Kraków 2009 Methods – Workpackages WP 5: Fusarium and mycotoxin analysis - Agrotest Fyto, Ltd., Kromeriz, Czech Republic, Ivana Polisenska (P15); P4, P6, P8  analysis of mycotoxin content (DON, T-2, HT-2): ELISA, LC-MS/MS  determination of Fusarium species: PCR

16. Owies, Kraków 2009 Fusarium on oats – basic facts  the symptoms of the infection on panicles in the field are only rarely observable (Gilbert and Tekauz, 2000)  newly described species F. langsethiae (Torp and Adler, 2004; Torp and Nirenberg, 2004) is quite often present on oats - no symptoms, T-2 and HT-2 production  Parrika et al. (2006) - in Finland: F. langsethiae, F. poae, F. culmorum, F. graminearum, F. avenaceum  Imathiu et al. (2006) - in GB: mainly F. langsethiae on oats  Kiecana and Perkowski; Kiecana and Mielniczuk (2001) – in Poland: F. avenaceum, F. culmorum, F. poae, F. sporotrichioides and F. crookwellense

17. Owies, Kraków 2009  AVEQ = Avena genetic resources for quality in human consumption Why DON, T-2 and HT-2 in AVEQ? DON ZEA (ppb) (ppb) unprocessed cereals other than durum wheat, oats and maize 1250 100 unprocessed durum wheat and oats 1750 100  DON and ZEA have the same producer – F.graminearum and F.culmorum

18. Owies, Kraków 2009 Why DON, T-2 and HT-2 in AVEQ?  T-2 and HT-2 are mentioned, but without limits - till now (31) …… the presence of T-2 and HT-2 toxin can be of concern for public health. Therefore, the development of a reliable and sensitive method, collection of more occurrence data and more investigations/research in the factors involved in the presence of T-2 and HT-2 toxin in cereals and cereal products, in particular in oats and oat products, is necessary and of high priority.

19. Owies, Kraków 2009 Fusarium and mycotoxins in AVEQ  Field trials, inoculation with F.culmorum, F.graminearum, F.sporotrichioides, F.langesethiae and F.avenaceum on 3 locations, in Italy natural infection  Observations in the field and on harvested material  Mycotoxin analysis – analytical problems: (ELISA vs. LC- MS/MS) – comparison study  Harmonisation of laboratories

20. inoculatednon -inoculated Owies, Kraków 2009 ELISA vs. LC-MS/MS, DON AVEQ standard cultivars

21. Owies, Kraków 2009 ELISA vs. LC-MS/MS, T-2 inoculatednon -inoculated AVEQ standard cultivars

22. Owies, Kraków 2009 Fusarium spp. on oat cv. Belinda

23. Owies, Kraków 2009  Analysis for protein (including Avenin patterns), fat and minerals Analytical WP: 6 - 8  Analysis for starch, fibre and ß- Glucan  Analysis for avenanthramides and tocols WP 9: Analysis for cold and drought tolerance - Italy, Romania

24. Owies, Kraków 2009 WP 10: Project documentation and Internet portal - Julius Kuehn Institute, Institute for Breeding Research on Agricultural Crops, Quedlinburg, Germany, Christoph U. Germeier (P0) http://eadb.bafz.de/aveq/ All results will be made available online in a crop portal for Avena based on the European Avena Database (EADB).

25. Owies, Kraków 2009 Thank you for your attention Acknowledgements  Peter Koelln KGaA, Elmshorn, Germany  Emco Ltd., Praha, Czech Republic  Gemeinschaft zur Förderung der privaten deutschen Pflanzenzüchtung e.V. (GFP), Bonn, Germany  European Commission, DG AGRI council regulation 870/2004.