1. Figure 1.1 Types of Microorganisms

2. Figure 1.1a

3. Figure 1.1b

4. Figure 1.1c

5. Figure 1.1d

6. Figure 1.1e

8. Figure 1.2

9. Figure 1.4

10. Nobel Prizes for Microbiology Research * The first Nobel Prize in Physiology or Medicine. 1901* von Behring Diphtheria antitoxin 1902 Ross Malaria transmission 1905 Koch TB bacterium 1908 Metchnikoff Phagocytes 1945 Fleming, Chain, Florey Penicillin 1952 Waksman Streptomycin 1969 Delbrück, Hershey, Luria Viral replication 1987 Antibody genetics 1997 Prusiner Prions 2005 Marshall & Warren H. pylori & ulcers

11. The Debate over Spontaneous Generation Spontaneous generation: The hypothesis that living organisms arise from nonliving matter; a “vital force” forms life Biogenesis: The hypothesis that the living organisms arise from preexisting life

12. ConditionsResults Three jars covered with fine net No maggots Three open jarsMaggots appeared From where did the maggots come? What was the purpose of the sealed jars? Spontaneous generation or biogenesis? Evidence Pro and Con 1668: Francesco Redi filled 6 jars with decaying meat

13. ConditionsResults Nutrient broth heated, then placed in sealed flask Microbial growth From where did the microbes come? Spontaneous generation or biogenesis? Evidence Pro and Con 1745: John Needham put boiled nutrient broth into covered flasks

14. ConditionsResults Nutrient broth placed in flask, heated, then sealed No microbial growth Spontaneous generation or biogenesis? Evidence Pro and Con 1765: Lazzaro Spallanzani boiled nutrient solutions in flasks

15. ConditionsResults Nutrient broth placed in flask, heated, not sealed Microbial growth Nutrient broth placed in flask, heated, then sealed No microbial growth Spontaneous generation or biogenesis? Evidence Pro and Con 1861: Louis Pasteur demonstrated that microorganisms are present in the air

16. Figure 1.3

17. The Golden Age of Microbiology 1857–1914 Beginning with Pasteur’s work, discoveries included the relationship between microbes and disease, immunity, and antimicrobial drugs

18. Fermentation and Pasteurization Pasteur showed that microbes are responsible for fermentation Fermentation is the conversion of sugar to alcohol to make beer and wine Microbial growth is also responsible for spoilage of food Bacteria that use alcohol and produce acetic acid spoil wine by turning it to vinegar (acetic acid)

19. Figure 1.4 Fermentation and Pasteurization Pasteur demonstrated that these spoilage bacteria could be killed by heat that was not hot enough to evaporate the alcohol in wine Pasteurization is the application of a high heat for a short time

20. Figure 1.4 The Germ Theory of Disease 1876: Robert Koch proved that a bacterium causes anthrax and provided the experimental steps, Koch’s postulates, to prove that a specific microbe causes a specific disease

21. Figure 14.3: Foundation Figure: Koch’s PostulatesSlide 2 Microorganisms are isolated from a diseased or dead animal. 1

22. Figure 14.3: Foundation Figure: Koch’s PostulatesSlide 3 Colony Microorganisms are isolated from a diseased or dead animal. 1 The microorganisms are grown in pure culture. 2a The microorganisms are identified.2b

23. Figure 14.3: Foundation Figure: Koch’s PostulatesSlide 4 Colony Microorganisms are isolated from a diseased or dead animal. 1 The microorganisms are grown in pure culture. 2a The microorganisms are injected into a healthy laboratory animal. 3 The microorganisms are identified.2b

24. Figure 14.3: Foundation Figure: Koch’s PostulatesSlide 5 Colony Microorganisms are isolated from a diseased or dead animal. 1 The microorganisms are grown in pure culture. 2a The microorganisms are injected into a healthy laboratory animal. 3 The disease is reproduced in a laboratory animal; microorganisms are isolated from this animal. 4 The microorganisms are identified.2b

25. Figure 14.3: Foundation Figure: Koch’s PostulatesSlide 1 Colony Microorganisms are isolated from a diseased or dead animal. 1 The microorganisms are grown in pure culture. 2a The microorganisms are injected into a healthy laboratory animal. 3 The disease is reproduced in a laboratory animal; microorganisms are isolated from this animal. 4 The microorganisms are grown in pure culture. 5a Identical microorganisms are identified. 5b The microorganisms are identified.2b

26. Figure 1.5

27. Figure 1.7

28. Figure 1.8

29. Figure 23.21 Ebola Hemorrhagic Fever Causes fever, hemorrhaging, and blood clotting First identified near Ebola River, Congo Outbreaks every few years