1. CCE project update Metabolomics Raftery Group

2. Original Study 20 cancer, 28 normals and 14 with polyps NMR and GC-MS study

3. CCE Samples and Experiments Healthy Control Colon Cancer Polyps Rectal Cancer unknown Sample number 461432414 Patient number 4610*32214 *9 sample were collected before/on the day of treatment/surgery  110 Samples received (104 patients)  1 H NMR experiment

4. 26 metabolites quantified; 8 showed p<0.05 between cancer and normal Unsupervised analysis on all samples showed little difference Supervised analysis in progress. Shows some separation but needs additional MS data  more cancer samples needed for better discrimination model. Multivariate analysis

5. Seven metabolite model built on previous colon sample (17 Cancer, 17 Healthy), predict on CCE samples (14 Cancer, 46 Healthy, 32 Polyps)  Q1: For those samples collected on the day of treatment, which were drawn before therapy or surgery?  Q2: Are CCE samples collected as the same way as previous samples? Looks like colon cancer samples are failing, but many were collected after surgery. Could be showing some treatment effect.