1. 954.502 (z=7) 956.785 (z=7) 956.787 (z=7) 959.073 (z=7) MSMS/MS Ba 7702 Bc AH819 Bc 10987 Bc AH259 b 26 3+ y 33 3+ y 55 5+ b 31 4+ b 9 2+ b 26 3+ y 33 3+ y 55 5+ b 31 4+ b 9 2+ b 26 3+ y 33 3+ y 55 5+ b 31 4+ b 9 2+ b 26 3+ y 33 3+ y 55 5+ b 31 4+ b 9 2+ Supplementary Figure S-1 A

2. B C

3. MSMS/MS Bc CA Supplementary Figure S-2 471.37 837.73 935.03 1111.21 1148.65 954.504 (z=7) 954.503 (z=7) 954.505 (z=7) b 26 3+ y 33 3+ y 55 5+ b 31 4+ b 9 2+ 471.31 837.79 935.32 1111.15 1148.42 b 26 3+ y 33 3+ y 55 5+ b 31 4+ b 9 2+ 471.39 837.64 934.88 1110.97 1148.36 b 26 3+ y 33 3+ y 55 5+ b 31 4+ b 9 2+ Bc CI Bc B06020

4. Supplementary Figure S-3 B. anthracis A

5. Supplementary Figure S-3 B. anthracis B

6. C Supplementary Figure S-3

7. B. anthracis D Supplementary Figure S-3

8. B. anthracis E Supplementary Figure S-3

9. B. anthracis F Supplementary Figure S-3

10. B. anthracis G Supplementary Figure S-3

11. B. anthracis H Supplementary Figure S-3

12. B. anthracis I Supplementary Figure S-3

13. B. anthracis J Supplementary Figure S-3 * * *

14. Supplementary Figure S-4 LVGGVAVIK ILDQSADK GTPDQEVIDQVLNVFK ARSTNKLAVPGAE SRSTNKLAVPGAE LVSLAEQQLGGFQK LVSLAEQQLGGYQK AKKAQASGASIQSTNASYGTE 2 min. 6.7 913.5 15.520 seg 1 seg 2 seg 3 seg 4seg 5

15. SASP-B (N-Ter peptide) SASP-B (C-Ter peptide) 30S ribosomal protein S10 SASP-gamma 60kDa Chaperonin Lipoprotein A B IS Mix 5 Bc IS Mix 5 Bc + 1 Ba (ratio 1:0.01) RT: 7.99 AA: 79072212 RT: 13.12 AA: 102613434 RT: 15.04 AA: 519440 RT: 15.01 AA: 12135726 RT: 8.13 AA: 98883827 RT: 13.10 AA: 116838072 Supplementary Figure S-5

16. SASP-gamma IS 693.5  966.2 Sample 690.9  962.3 0 : 1 Ratio Ba : Bc mix 0.01 : 10.1 : 1 1 : 1 Supplementary Figure S-6 0.01 : 10.1 : 1 1 : 1 A B