1. Swimmer’s Itch Survey 2015 Volunteer Sampling Protocols

2. Aims of Survey 1.Temporal surveys: Obtain field data that can be used to validate models predicting short term fluctuations in swimmer’s itch exposure. July 6, 2015 – August 2, 2015 2. Spatial Survey: Collaborate with volunteers to obtain data from 8 lakes to determine predictors of schistosome cercariae abundance across the region Each site will be visited twice throughout project by Maddie in order to survey snail populations and collect habitat assessment variables.

3. What determines swimmer’s itch exposure? Snail population density Percent snails infected Cercariae produced per snail Bird infection Temperature variation Nutrient loading (Eutrophication) Cercariae in water SWIMMER’S ITCH!

4. Location of Sites LakeCountyNumber of SitesWatershed Crystal LakeBenzie4Betsie-Platte Platte LakeBenzie1Betsie-Platte Glen LakeLeelanau3Betsie-Platte Little Traverse LakeLeelanau1Betsie-Platte Lime LakeLeelanau1Betsie-Platte Lake LeelanauLeelanau2Betsie-Platte Walloon LakeCharlevoix1Boardman-Charlevoix Higgins LakeRoscommon3Muskegon

5. Defining the site All water sampling will be conducted in the littoral zone Riparian zone will be used to characterize land use and habitat at site Drawdown zone is negligible and can be ignored when lake is at normal water mark

6. Daily Sampling: Supplies Suggested supplies (not provided): Waders Wind meter (way to measure speed and/or direction) Thermometer (air temp) Binoculars (bird sighting) Meter stick (depth) Watch/ stopwatch Provided: Clipboard Daily datasheets & folder Nitex filter scoop 15 mL centrifuge tubes (30) 70% ethanol squirt bottle (red) Water squirt bottle (white) 4 brown HDPE sample bottles Writing utensils

7. Launching Temp Loggers Programmed to start logging at 12 am July 6 – Place temp loggers in water before midnight on July 5 th “A”: deep logger, monitors temp and light intensity. – place within littoral zone 50-100 cm deep. Cinder block should have marker buoy. Do not place farther than 10 m from shoreline. Record depth of logger on site assessment data sheet. “B”: shallow logger, monitors only temp. – Place within littoral zone 25-50 cm deep near shoreline. Record depth of logger on site assessment data sheet.

8. Collecting Daily Filter Samples 1.Fill out top of daily field data sheet as much as possible 2.Label one 15 mL centrifuge tube 3.Enter water near left boundary of site (wear waders if possible) 4.Fill 1-liter container to the brim and pour into filter let water drain through 5.Repeat step 3 until you have filled and drained the filter a total of 50 times while stepping to the right along the shoreline. You may zig zag in and out to various depths within the littoral zone. 6.While collecting filter samples run through checklist on bottom of daily field data sheet and make note of any bird sightings. 7.Exit the water 8.Take cap off 15 mL tube, place funnel in top of tube 9.Use water squirt bottle to concentrate the contents of the filter into one corner. Do not let filter contents spill out 10.Use ethanol squirt bottle to rinse filter contents into funnel and into 15 mL tube. Do not overfill tube. 11.Secure cap on tube and store in cool dark place. 12.Fill out rest of daily field data sheet 13.Optional: conduct sampling again, rinse filter into tube with water, add Lugol’s iodine to tube for microscopic observation

9. Collecting Daily Filter Samples SITE ID6/26/15 LABELLING 15 mL TUBES Clearly print with permanent marker the site ID horizontally on the tube so that it can be read from left to right Skip a space and then write the date in number form: M/DD/YY Site ID Crystal- CCLee-N Crystal-OHLee-S Crystal-CSATraverse Crystal-OnkLime PlatteWalloon Glen-DHiggins-1 Glen-KHiggins-2 Glen-LHiggins-3

10. Contact Info Call Maddie Messner if you need more supplies, have questions, concerns, or if you cannot get the sampling taken on a particular day. Cell: 440-344-9858 email: mlmessner@oakland.edu mlmessner@oakland.edu Maddie will need to access the site at various points throughout survey so please coordinate with her if this will be an issue at any point.