1. Culturing BacteriaBacteria

2. Lab Preparation In this lab you will be designing an experiment to test the effectiveness of a product that contains an antibacterial chemical.

3. Question What are you going to test in this experiment?

4. Make an INFORMED prediction. To be INFORMED you need background info: - Ingredients in each product -Claims made by the manufacturer Must be written in IF…THEN… format IF (Manipulated variable) THEN (Responding variable)

5. Manipulated, Responding, Controlled variables

6. Experimental control group vs test group

7. Preparing Petri dish Divide the BOTTOM of the dish into 3 or 4 sections depending on the number of products you are testing. Write your initials and class period on the SIDE of the LID.

8. Swabbing a sample Use the sterile swab and slide it across the sample area 2-3 times. If you are testing: Hand sanitizer: swab your fingers/hands Mouthwash: swab the inside of your cheeks Disinfectant: swab a surface that you would use the disinfectant on

9. Create the LAWN Gently rub the swab across the surface of your agar

10. Storing your Petri dish Tape your lid down Place in incubator

11. Zone of Inhibition Zone of inhibition The size of the ZONE determines the effectiveness of the antibiotic.