1. FITOP 8.67 AS AN ECO-FRIENDLY MEANS TO CONTROL GROWTH, DEVELOPMENT AND PRODUCTIVITY IN PLANTS “Research Center” 2014

2. In modern agriculture one of the acute problems is a complex stress caused by adverse environment factors in cultivated plants:  soil and climate;  adverse weather conditions;  biotic factor (phytopathogens, pests, weed vegetation);  anthropogenic factor (intensive agricultural use, environmental pollution). Prophylaxis to prevent stress and diseases requires advanced methods which contribute to arranging conditions for optimal need satisfaction in cultivated plants and enable us to manage the processes of plant growth, development and immunity formation. Fitop 8.67 is a modern biologically multifunctional preparation with a complex effect on cultivated plants, soil and detrimental organisms. The preparation was developed on the base of saprophytic bacteria Bacillus subtilis (Ehrenberg) Cohn, Bacillus amyloliquefaciens (Fukumoto) Priest et al. by Scientific and Production Company “Issledovatelsky Tsentr” (“Research Center”) in Koltsovo Science Town, Novosibirsk region, Russia. The composition of the preparation includes three strains: Bacillus subtilis VKPM B-10641 (DSM 24613) and Bacillus amyloliquefaciens VKPM B-10642 (DSM 24614) and В-10643 (DSM 24615) in equal parts. They were isolated from the soil of the Siberian environmentally pristine areas and selected by the developer. Now they are deposited in the Russian Collection of Industrial Microorganisms (VKPM) and the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH European collection of microorganisms and cell cultures (DSMZ). Fitop 8.67

3. Intended use of Fitop 8.67 The preparation Fitop 8.67 is applied uppermost at the earlier technological stages and serves as a means to cultivate young plants owing to the direct impact of bacteria on the plant body and environment of this plant. The environmental changes happen owing to antagonistic effect on plant pathogens, stabilizing effect on soil microbial community and enhancement of soil suppressivity. Basic ways of Fitop 8.67 application to treat plants and soil :  presowing seed treatment;  preplanting cutting treatment;  watering of the soil with the Fitop solution after sowing or after emerging seedlings;  steeping of the root system of seedlings in the Fitop solution;  Fitop drip or furrow irrigation of the root system of young plants transplanted in permanent place. In addition, the preparation Fitop 8.67 can be applied as antistress agent to treat the above-ground parts of plants at vegetative stage after herbicidal treatment and as a means of controlling infectious diseases due to the antagonistic effect of the bacteria on phytopathogens. Tests and application of Fitop 8.67: The long-term production tests with Fitop 8.67 and field experiments in grains, potatoes, vegetables, fruits, berries and flowers in the different climatic zones of our country and abroad (Italy, Spain, Kazakhstan) were carried out by scientists and manufacturers. The following effects are observed: - stimulating the root system length and biomass (up to 15-20%); - increasing the total plant biomass (20-25% and higher); - increasing the productivity (20-30%). The preparation increases survival rate in plants under drought conditions and relieves shock states after herbicidal treatments. The active process of immunization in the treated plants with Fitop and posterior decrease in various crops affected by infectious spread through the soil, seeds and droplet way was proved. The results of the experiments conducted in China and Vietnam in 2014 are presented below.

4. Variants Difference vs. the control (organs) Increase vs. the control, % Fitop 8.67 2.72.741.3 Fitop 8.67 + fertilizer -0.1-2.2 The effect of double treatment with Fitop 8.67 on the length of above-ground part in the plant of strawberry (experimental farm Tongzhou, March 24, 2014) Planting in the greenhouse : August 23, 2013. Consumption: 50 liters of the solution per 310 m 2. Fitop 8.67 application: drip irrigation after transplanting n the permanent place and at the early stage of budding VariantsIncrease vs. the control, (leaves) Increase vs. the control, % Fitop 8.67 3.119.0 Fitop 8.67 + fertilizer 0.50.52.92.9

5. Difference vs. the control Increase vs. the control, % 5.122.8 Difference vs. the control Increase vs. the control, % 1.51.515.2 Difference vs. the control Increase vs. the control, % 1.61.638.6 Fitop 8.67 treatment effect on the above-ground part length, the number of internodes and reproductive organs in the plant of hot pepper after transplanting in permanent place (Lufulon farm, Yanqing area, March 25, 2014) Planting in the greenhouse: February 17, 2014. Fitop 8.67 application: drip irrigation, February 19, 2014

6. Fitop 8.67 treatment effect on the plant of hot pepper after transplanting in permanent place (Lufulon farm, Yanqing area, March 25, 2014) Planting in the greenhouse: February 17, 2014 Fitop 8.67 application: drip irrigation, February 19, 2014 Control Fitop 8.67

7. Difference vs. the control Increase vs. the control, % 2.12.18.68.6 Difference vs. the control Increase vs. the control, % 0.80.88.08.0 Difference vs. the control Increase vs. the control, % 1.11.127.4 Fitop 8.67 treatment effect on the above-ground part length, the number of internodes and reproductive organs in the plant of sweet pepper after transplanting in permanent place (Lufulon farm, Yanqing area, March 25, 2014. Planting in the greenhouse: February 17, 2014 Fitop 8.67 application: drip irrigation, February 19, 2014

8. Control Fitop 8.67 Fitop 8.67 treatment effect on the plant of sweet pepper after transplanting in permanent place (Lufulon farm, Yanqing area, March 25, 2014) Planting in the greenhouse: February 17, 2014 Fitop 8.67 application: drip irrigation, February 19, 2014

9. Difference vs. the control Increase vs. the control, % 7.17.113.0 Difference vs. the control Increase vs. the control, % 2.42.417.2 Difference vs. the control Increase vs. the control, % 1.01.043.6 Fitop 8.67 treatment effect on the above-ground part length, the number of internodes and reproductive organs in the plant of tomato after transplanting in permanent place (farm Lufulon, Yanqing area, March 25, 2014) Planting in the greenhouse: February 17, 2014 Fitop 8.67 application: drip irrigation, February 19, 2014

10. Difference vs. the control Increase vs. the control, % 18.548.3 Difference vs. the control Increase vs. the control, % 0.90.929.1 The effects of water treatment with Fitop 8.67 of seedlings in cassettes on the length of the stem and the number of true leaves in the plant of tomato (farm in Zhouzhou, Boadin City, March 26, 2014) Sowing of seeds in cassettes: February 14, 2014 Fitop 8.67 application: in 5 days after emerging seedlings, February 24, 2014

11. The effect of water treatment with Fitop 8.67 of seedlings in cassettes on the stem length and the number of true leaves in the plant of tomato (farm in Zhouzhou, Boadin City, March 26, 2014) Sowing seeds in cassettes: February 14, 2014 Fitop 8.67 application: in 5 days after emerging seedlings, February 24, 2014) Control Fitop 8.67

12. Difference vs. the control Increase vs. the control, % 10.510.3 Difference vs. the control Increase vs. the control, % 0.4612.9 The effect of water treatment with Fitop 8.67 of seedlings in cassettes on the length of the above-ground part and the number of true leaves in the plant of Broccoli (farm in Zhouzhou, Boadin City, March 26, 2014) Sowing seeds in cassettes: February 14, 2014 Fitop 8.67 application: in 5 days after emerging seedlings, February 24, 2014

13. The effect of water treatment with Fitop 8.67 of seedlings in cassettes on the length of the above-ground part and the number of true leaves in the plant of Broccoli (farm in Zhouzhou, Boadin City, March 26, 2014) Sowing seeds in cassettes: February 14, 2014 Fitop 8.67 application: in 5 days after emerging seedlings, February 24, 2014 Fitop 8.67 Control

14. Difference vs. the controlIncrease vs. the control, % 3.53.516.5 Difference vs. the controlIncrease vs. the control, % 0.60.615.4 The effect of seed treatment and watering the plant roots with Fitop 8.67 on the length of the above-ground part and the number of true leaves in the plant of asparagus haricot (farm in Hoa Binh, Hanoi suburb, April 26, 2014) Seed sowing : April 3, 2014 Fitop 8.67 application: watering in plant roots, April 10-15, 2014

15. Difference vs. the control Increase vs. the control, % 3.93.921.1 Difference vs. the control Increase vs. the control, % 0.80.817.9 Difference vs. the control Increase vs. the control, % 1.71.753.9 Fitop 8.67 treatment effect on the length of the above-ground part, root length and the number of true leaves in the plant of Chinese cabbage -Pak choi (farm in Tien Le, Hanoi suburb, April 26, 2014)

16. The effect of seed treatment with Fitop 8.67 on the length of the above-ground part, root length (cm) and the number of true leaves in the plant of Chinese cabbage - Pak choi (farm Tien Le, Hanoi suburb, April 26, 2014) Fitop 8.67 Control Seed sowing: April 4, 2014

17. Difference vs. the control Increase vs. the control, % 3.13.120.5 Difference vs. the control Increase vs. the control, % 1.51.529.2 Difference vs. the control Increase vs. the control, % 1.41.436.1 The effect of seed treatment with Fitop 8.67 on the length of the above-ground part, root length (cm) and the number of true leaves in spinach (farm Tien Le, Hanoi suburb, April 26, 2014) Seed sowing: April 4, 2014

18. The effect of seed treatment with Fitop 8.67 on the length of the above-ground part, root length (cm) and the number of true leaves in spinach (farm Tien Le, Hanoi suburb, April 26, 2014) Seed sowing: April 4, 2014 Fitop 8.67 Control

19. Difference vs. the control Increase vs. the control, % 4.326.2 Difference vs. the control Increase vs. the control, % 1.329.1 Difference vs. the control Increase vs. the control, % 0.723.5 The effect of seed treatment with Fitop 8.67 on the length of the above-ground part, root length (cm) and the number of true leaves in the plant of celery cabbage – Choi Sum (farm Tien Le, Hanoi suburb, April 26, 2014) Seed sowing: April 4, 2014

20. The effect of seed treatment with Fitop 8.67 on the length of the above-ground part, root length (cm) and the number of true leaves in the plant of celery cabbage – Choi Sum (farm Tien Le, Hanoi suburb, April 26, 2014) Fitop 8.67 Control Seed sowing: April 4, 2014

21. Difference vs. the control Increase vs. the control, % 12.338.7 Difference vs. the control Increase vs. the control, % 0.70.712.1 The effect of seed treatment with Fitop 8.67 on the length of the above-ground part and the number of true leaves in water spinach – Kang kong (company An Viet, Hanoi suburb, April 26, 2014) Seed sowing: April 4, 2014

22. The effect of seed treatment with Fitop 8.67 on the length of the above-ground part and the number of true leaves in water spinach Kang kong (company An Viet, Hanoi suburb, April 26, 2014) Seed sowing: April 4, 2014 Water spinach Kang kong Fitop 8.67 Control

23. Difference vs. the control Increase vs. the control, % 6.137.2 Difference vs. the control Increase vs. the control, % 2.027.9 The effect of seed treatment with Fitop 8.67 on the length of the above-ground part and the number of true leaves in the plant of daikon – Radish (firm Phy An, Hanoi suburb, April 26, 2014) Seed sowing: April 3, 2014

24. Difference vs. the control Increase vs. the control, % 6.060.8 Difference vs. the control Increase vs. the control, % 1.116.4 The effect of seed treatment with Fitop 8.67 on the length of the above-ground part and the number of true leaves in the plant of amaranth (firm Linh Nam, Hanoi suburb, April 27, 2014) Seed sowing: April 4, 2014

25. The effect of seed treatment with Fitop 8.67 on the length of the above-ground part and the number of true leaves in the plant of amaranth (firm Linh Nam, Hanoi suburb, April 27, 2014) Seed sowing: April 4, 2014 Fitop 8.67 Control

26. Difference vs. the control Increase vs. the control, % 3.43.436.2 Difference vs. the control Increase vs. the control, % 1.01.028.3 The effect of seed treatment with Fitop 8.67 on the length of the above-ground part and the number of true leaves in Yellow spinach (firm Dang Xa, Hanoi suburb, April 27, 2014) Seed sowing: April 4, 2014

27. Difference vs. the control Increase vs. the control, % 3.13.114.9 Difference vs. the control Increase vs. the control, % 0.90.921.9 The effect of seed treatment with Fitop 8.67 on the length of the above-ground part and the number of true leaves in the plant of cucumber (Fruit and Vegetable Research Institute, Hanoi suburbs, April 28, 2014 ) Transplantation in permanent place: April 10, 2014

28. The effect of seed treatment with Fitop 8.67 on the length of the above-ground part and the number of true leaves in the plant of cucumber (Fruit and Vegetable Research Institute, Hanoi suburb, April 28, 2014 ) Transplantation in permanent place: April 10, 2014 Control Fitop 8.67

29. Difference vs. the control Increase vs. the control, % 0.90.929.7 Difference vs. the control Increase vs. the control, % 0.70.733.6 The effect of seed treatment with Fitop 8.67 on the length of the above-ground part and the number of true leaves in the plant of Cleona (University of Agricultural, Hanoi, April 28, 2014 ) Sowing into cassettes: April 3, 2014

30. The effect of seed treatment with Fitop 8.67 on the length of the above-ground part and the number of true leaves in the plant of Cleona (University of Agricultural, Hanoi suburb, April 28, 2014 ) Sowing into cassettes: April 3, 2014 Control Fitop 8.67

31. Difference vs. the control Increase vs. the control, % 3.43.432.5 Difference vs. the control Increase vs. the control, % 1.41.424.6 The effect of seed treatment with Fitop 8.67 on the length of the above-ground part and the number of true leaves in the plant of Cosmos (Elite area of Hanoi, April 28,2014) Sowing into cassettes: April 3, 2014

32. Difference vs. the control Increase vs. the control, % 1.155.0 Difference vs. the control Increase vs. the control, % 0.513.9 Difference vs. the control Increase vs. the control, % 0.634.7 Vinca The effect of seed treatment with Fitop 8.67 on the length of the above-ground part and the number of true leaves in the plant of Button poms and Vinca (Elite area of Hanoi, April 28,2014) Sowing into cassettes: April 4, 2014 Button poms

33. CosmosVinca Контроль The effect of seed treatment with Fitop 8.67 on the length of the above-ground part and the number of true leaves in the plants of Cosmos and Vinca (Elite area of Hanoi, April 28,2014) Sowing into cassettes: April 3, 2014 Button poms Control Fitop 8.67