1. Microbial for ACP Management Cooperative Work Plan between NCSU/CIPM & FAAS/ABRRI (2010-2011)

2. 1.Introduction 2.Work plan 3.Foundation Presentation Content

3. Introduction Damage of Huanglongbing (HLB) Damage of Huanglongbing (HLB) Citrus Huanglongbing Damage of Huanglongbing

4. Introduction Vector of Huanglongbing Vector of Huanglongbing Transmitting HLB Diaphorina citri (ACP)

5. Introduction Control of ACP Controlling ACP is a main and effective approach to manage HLB

6. Introduction Advancement of ACP Control Chemical control  Conventional approach approach  Usually undesirable  Many side effects

7. Introduction Advancement of ACP Control Biological control Environmentally Environmentally friendly friendly Few biological Few biological agents studied agents studied No biological No biological agents used in agents used in practice of ACP practice of ACP control control

8. Introduction Potential of microbial for bio-control of ACP 1.Microbial are resources of biological agents against pest insects against pest insects Biological pesticides Gene resource for GMP

9. Introduction Potential of microbial for bio-control of ACP 2. Reported microbial against ACP Cephalosphorium lecanii Zimm Isaria fumosorosea Wize

10. Work plan Objective Find new microbial for ACP management

11. Work plan Content 1.Occurrence of ACP microbial parasites in field 2.Isolation and reconfirmation of ACP microbial parasites 3.Identification of ACP microbial parasites 4.Biology of ACP microbial parasites 5.Production technology of ACP microbial parasites 6.Evaluation of the efficiency of ACP microbial parasites

12. Work plan 1.Occurrence of ACP microbial parasites in field 1.1 Population dynamic of ACP 1.2 Occurrence of ACP parasites 1.3 Contribute of ACP parasites to regulating ACP population 1.4 Collection of ACP parasites from field Timeline: 2010/04-2011/04

13. Work plan 2. Isolation and reconfirmation of ACP microbial parasites 2.1 Rearing ACP for bioassay 2.2 Isolating and reconfirming microbial pathogenic against ACP 2.2.1 Microbial from infected ACP 2.2.2 Microbial inhabiting on plant surface surface 2.2.3 Microbial endophyte inner plant 2.2.4 Microbial from soil Timeline: 2010/04-2010/12

14. Work plan 3. Identification of ACP microbial parasites 3.1 Fungi pathogenic against ACP 3.2 Bacteria pathogenic against ACP 3.3 Virus pathogenic against ACP (Send to taxonomist for identification) (Send to taxonomist for identification) Timeline: 2010/04-2010/12

15. Work plan 4. Biology of ACP microbial parasites 4.1 Climatic condition for microbial growth 4.2 Nutrition condition for microbial growth 4.3 Effects of climatic condition on microbial pathogenicity 4.4 Effects of nutrition medium on microbial pathogenicity Timeline: 2010/08-2011/03

16. Work plan 5. Production technology of ACP microbial parasites 5.1 Fermentation condition Medium formula, temperature, inoculum, rotation rate, etc. Medium formula, temperature, inoculum, rotation rate, etc. 5.2 Formation Timeline: 2010/04-2011/06

17. Work plan 6. Evaluation of the efficiency of ACP microbial parasites 6.1 Greenhouse experiments 6.2 Field experiments Timeline: 2010/06-2011/12

18. Work plan Deliverable results 1.Report of population dynamic of ACP and the natural occurrence of its microbial parasites 2.Isolation of 2-3 microbial strains against ACP

19. Work plan Budget for this year Personel (salary etc): 7400 USD Materials: 3000 USD Travels: 4000 USD Eletricity energy: 1600 USD Collaborator: 3000 USD Overhead: 1000 USD Sum: 20000 USD

20. Personal: We need to pay our students and scientists working on the project Tow graduate students, 150 USD/month, 10 month for work, total 3000 USD tow months of a senior scientist, 1000 USD/month, total 2000 USD One full time research assistant, 200 USD/month, 12 month for work, total 2400 USD Sum 7400 USD Work plan Budget Narrative 1

21. Materials: Purchasing citrus seedlings and culturing pots to rear Asian Citrus Psyllid, 1500 USD Purchasing medium, Petri discs for culturing microbials, 1500 USD Sum 3000 USD Travels: Travel to citrus orchards in different regions for investigation and sampling, 4000 USD Spending of eletricity energy: 1600 USD Work plan Budget Narrative 2

22. Collaborator: Payment for citrus farmer for their information and offering citrus orchard for experiment, 1000 USD Identification of entomopathogens of Asian Citrus Psyllid, 2000 USD Sum 3000 USD Overhead: The fund for the institute to maintain the facilities on good condition for conducting experiments, 1000 USD Work plan Budget Narrative 3

23. Foundation 1.Long history of study on Huanglongbing and its vector its vector Morphology of HLB PathogenMorphology of HLB Pathogen Pathology and epidemiology of HLBPathology and epidemiology of HLB Biology, ecology and control of ACPBiology, ecology and control of ACP

24. Foundation 1.Long history of study on Huanglongbing and its vector its vector Morphology of HLB PathogenMorphology of HLB Pathogen Pathology and epidemiology of HLBPathology and epidemiology of HLB Biology, ecology and control of ACPBiology, ecology and control of ACP

25. Foundation 2.Profound research and utilization of microbial 2.1 Collection center of microbial resource >5000 soil samples from different regions over the world >10000 Bacillus strains

26. Fertilizer Pesticide Feedstuff Clarifier Preservative Bacillus A R&D Center Foundation 2.Profound research and utilization of microbial 2.2 Utilization of microbial

27. Foundation 2.Profound research and utilization of microbial 2.3 Research projects 2.3.1 Study on Bio-insecticide BtA

28. Foundation 2.Profound research and utilization of microbial 2.3 Research projects 2.3.2 Study on Bio-bactericide strain ANTI-8098A against Ralstonia solanacearum of vegetables against Ralstonia solanacearum of vegetables

29. Foundation 2.Profound research and utilization of microbial 2.3 Research projects 2.3.3 Study on Bio-preservative for fruit Long’an

30. Bio-preservative for fruit Long’an

31. Foundation 3. Condition for research

32. Foundation 4. Research group

33. Foundation 4. Publications

34. Thank you for your attention ! Wellcome to China !