1. Abstraction Lesson 2 presented by Dr. Natalie Kuldell

2. Abstraction Hierarchy a human invention designed to assist people in engineering complex systems Sequences of DNA encode “parts”

3. “Part” – sequence of DNA with human defined function AAAATGCACCCGCTGTCGATCAAACGCGCGGTGGCGAATATGGTGGTCAACGCCGCCCGT TATGGCAATGGCTGGGTCAAAGTCAGCAGCGGAACGGAGCCGAATCGCGCCTGGTTCCA GGTGGAAGATGACGGTCCGGGAATTGCGCCGGAACAACGTAAGCACCTGTTCCAGCCGT TTGTCCGCGGCGACAGTGCGCGCACCATTAGCGGCACGGGATTAGGGCTGGCAATTGTGC AGCGTATCGTGGATAACCATAACGGGATGCTGGAGCTTGGCACCAGCGAGCGGGGCGGG CTTTCCATTCGCGCCTGGCTGCCAGTGCCGGTAACGCGGGCGCAGGGCATGACAAAAGA AGGGTAATCTAGAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGT TTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGCC

4. “Part” – sequence of DNA with human defined function AAAATGCACCCGCTGTCGATCAAACGCGCGGTGGCGAATATGGTGGTCAACGCCGCCCGT TATGGCAATGGCTGGGTCAAAGTCAGCAGCGGAACGGAGCCGAATCGCGCCTGGTTCCA GGTGGAAGATGACGGTCCGGGAATTGCGCCGGAACAACGTAAGCACCTGTTCCAGCCGT TTGTCCGCGGCGACAGTGCGCGCACCATTAGCGGCACGGGATTAGGGCTGGCAATTGTGC AGCGTATCGTGGATAACCATAACGGGATGCTGGAGCTTGGCACCAGCGAGCGGGGCGGG CTTTCCATTCGCGCCTGGCTGCCAGTGCCGGTAACGCGGGCGCAGGGCATGACAAAAGA AGGGTAATCTAGAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGT TTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGCC

5. “Part” – sequence of DNA with human defined function AAAATGCACCCGCTGTCGATCAAACGCGCGGTGGCGAATATGGTGGTCAACGCCGCCCGT TATGGCAATGGCTGGGTCAAAGTCAGCAGCGGAACGGAGCCGAATCGCGCCTGGTTCCA GGTGGAAGATGACGGTCCGGGAATTGCGCCGGAACAACGTAAGCACCTGTTCCAGCCGT TTGTCCGCGGCGACAGTGCGCGCACCATTAGCGGCACGGGATTAGGGCTGGCAATTGTGC AGCGTATCGTGGATAACCATAACGGGATGCTGGAGCTTGGCACCAGCGAGCGGGGCGGG CTTTCCATTCGCGCCTGGCTGCCAGTGCCGGTAACGCGGGCGCAGGGCATGACAAAAGA AGGGTAATCTAGAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGT TTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGCC

6. Parts

7. Abstraction Hierarchy a human invention designed to assist people in engineering complex systems Sequences of DNA encode “parts” Assemblies of parts make up devices Assemblies of devices make a system

8. Abstraction Hierarchy a human invention designed to assist people in engineering complex systems

9. Parts make devices

10. Abstraction: 4-Parts to make “NOT” gate

11. Slide from Nathan Hilson, JBEI Building biology from parts is a new way to engineer biotechnologies

12. Abstraction Hierarchy a human invention designed to assist people in engineering complex systems Everyone has to do their job right for the final product to work No single person understands everything about the final product Interfaces between layers are critically important

13. Tools that make biology easier to engineer PCR Genetic Engineering Synthetic Biology Sequencing rDNA Synthesis Abstraction Standardization A+A+C+T+T…

14. Putting Abstraction to Work: Choose your System

15. Abstraction Lesson 2 presented by Dr. Natalie Kuldell