1. Implementation of tetra- poly(ethylene glycol) hydrogel with high mechanical strength into microfluidic device technology Richard Welter

2. Introduction Polymeric materials used to form structural body PDMS 1 Hydrogel integration can extend microfluidic device. 1: Ocvirk, G.; Munroe, M.; Tang, T.; Oleshuk, R.; Westra, K.; Harrison, D. J. Electrokinetic control of fluid flow in native poly(dimethylsiloxane) capillary electrophoresis devices. Electrophoresis. 2000, 21, 107-115.

3. Hydrogel 2 Numerous setbacks tetra-poly(ethylene glycol) (tetra-PEG) Hydrogel Reactive Microbonding (HRMB) method Fluid flow Takehara, H.; Nagaoka, A.; Noguchi, J.; Akagi, T.; Sakai, T.; Chung, U.; Kasai, H.; Ichiki, T. Implementation of tetra-poly(ethylene glycol) hydrogel with high mechanical strength into microfluidic device technology. Biomicrofluidics. 2013, 7, 1-11.

4. HRMB Method 2 Gelation made from mixing TAPEG and TNPEG Mixture of TAPEG and TNPEG bonds PDMS and tetra-PEG Takehara, H.; Nagaoka, A.; Noguchi, J.; Akagi, T.; Sakai, T.; Chung, U.; Kasai, H.; Ichiki, T. Implementation of tetra- poly(ethylene glycol) hydrogel with high mechanical strength into microfluidic device technology. Biomicrofluidics. 2013, 7, 1-11.

5. Fluid Flow 2 Phosphate-buffered saline (PBS) Flow velocity measured via Fluorescence Correlation Spectroscopy (FCS) Takehara, H.; Nagaoka, A.; Noguchi, J.; Akagi, T.; Sakai, T.; Chung, U.; Kasai, H.; Ichiki, T. Implementation of tetra- poly(ethylene glycol) hydrogel with high mechanical strength into microfluidic device technology. Biomicrofluidics. 2013, 7, 1-11.

6. Results 2 HRMB successful with high reproducibility Bond interface not broken via Bulge Test Successful isolation in microchamber Smaller the pores in microchannel = less leakage FCS showed similar experimental and simulated results ∴ perfection of fabrication via HRMB Takehara, H.; Nagaoka, A.; Noguchi, J.; Akagi, T.; Sakai, T.; Chung, U.; Kasai, H.; Ichiki, T. Implementation of tetra-poly(ethylene glycol) hydrogel with high mechanical strength into microfluidic device technology. Biomicrofluidics. 2013, 7, 1-11.

7. Conclusion Fabrication successful via HRMB Analysis of flow velocity distribution Applicable to future research in tissue engineering and drug screening