1. The Cell Cycle

2. Overview What are the various stages of the cell cycle? What are the various stages of the cell cycle?

3. DNA Replication What is meant by semiconservative replication and how What is meant by semiconservative replication and how did Meselson and Stahl demonstrate that is is what took place? did Meselson and Stahl demonstrate that is is what took place?

4. DNA Replication What is theta replication and where does it take place? What is theta replication and where does it take place?

5. DNA Replication What are replicons? What are replicons? What is an origin recognition complex? What is an origin recognition complex? What is an autonomously replicating sequence? What is an autonomously replicating sequence?

6. DNA Replication What components are needed for DNA replication? What components are needed for DNA replication? What is the function of DNA polymerase? What is the function of DNA polymerase? In which directions does DNA synthesis occur? In which directions does DNA synthesis occur?

7. DNA Replication

8. What are Okazaki fragments? What are Okazaki fragments? What is the function of DNA ligase? What is the function of DNA ligase?

9. DNA Replication How are mistakes in DNA corrected? How are mistakes in DNA corrected?

10. DNA Replication What role does RNA play in DNA synthesis? What role does RNA play in DNA synthesis? What is primase? What is primase?

11. DNA Replication What is involved in unwinding the double helix in preparation for replication? What is involved in unwinding the double helix in preparation for replication?

12. DNA Replication

15. What is meant by the end-replication problem? What is meant by the end-replication problem?

16. DNA Replication How do eukaryotic cells handle the end- replication problem? How do eukaryotic cells handle the end- replication problem? What is meant by DNA licensing? What is meant by DNA licensing?

17. DNA Damage and Repair What kinds of spontaneous mutations regularly alter DNA molecules? What kinds of spontaneous mutations regularly alter DNA molecules? What kind of mutagens alter DNA molecules? What kind of mutagens alter DNA molecules?

18. DNA Damage and Repair What are the different types of repair mechanisms used to correct alterations in DNA? What are the different types of repair mechanisms used to correct alterations in DNA?

19. Nuclear and Cell Division

22. What is the difference between kinechore and polar microtubules? What is the difference between kinechore and polar microtubules?

23. Nuclear and Cell Division What influences the length of spindle fibers? What influences the length of spindle fibers?

24. Nuclear and Cell Division What is responsible for the alignment of chromosomes during metaphase? What is responsible for the alignment of chromosomes during metaphase? What is involved in the splitting of the chromatids? What is involved in the splitting of the chromatids?

25. Nuclear and Cell Division What kinds of motor proteins are involved in chromosome movement? What kinds of motor proteins are involved in chromosome movement?

26. Nuclear and Cell Division How does cytokinesis differ in animals and plants? How does cytokinesis differ in animals and plants?

27. Regulation of Cell Cycle What are the cell cycle checkpoints? What are the cell cycle checkpoints?

28. Regulation of Cell Cycle What is MPF and how do we know it exists? What is MPF and how do we know it exists?

29. Regulation of Cell Cycle How were mutant yeast cells used to find the molecule that controlled the cell cycle? How were mutant yeast cells used to find the molecule that controlled the cell cycle? What kind of protein is produced by the cdc 2 gene? What kind of protein is produced by the cdc 2 gene? What are cyclins? What are cyclins?

30. Regulation of Cell Cycle What are mitotic cyclins? What are mitotic cyclins?

31. Regulation of Cell Cycle Besides the availability of cyclins, what else controls the activity of MPF? Besides the availability of cyclins, what else controls the activity of MPF?

32. Regulation of Cell Cycle How does the active MPF bring about events of mitosis intiation? How does the active MPF bring about events of mitosis intiation?

33. Regulation of Cell Cycle How is G1 checkpoint controlled? How is G1 checkpoint controlled?

34. Regulation of Cell Cycle How does anaphase promoting complex control spindle assembly checkpoint? How does anaphase promoting complex control spindle assembly checkpoint? What ensures the proper timing of these events? What ensures the proper timing of these events?

35. Regulation of Cell Cycle

36. Cell Growth and Cancer How is the ras pathway involved in the cell cycle control? How is the ras pathway involved in the cell cycle control?

37. Cell Growth and Cancer How can growth factors inhibit cell division? How can growth factors inhibit cell division? Alterations in what kinds of genes lead to development of cancer? Alterations in what kinds of genes lead to development of cancer? How do oncogenes cause cancer? How do oncogenes cause cancer?

38. Cell Growth and Cancer How does the p53 tumor suppressor gene work? How does the p53 tumor suppressor gene work?