1. Basics of Chromatography Separation of Analytes Type of Equilibrium

2. Basics of Chromatography Ability to dissolve in mobile phase Interaction with stationary phase Time on X axis Response on Y

3. Chromatography Types

4. Separation dependent on time on the column (along with other factors)

5. Retention time of the analyte Retention time of the Mobile Phase

6. Basics of Chromatography Average linear flow rate Average linear flow of mobile phase

7. Plate Height Theoretical Measure of Efficiency

8. Fig. 26-6 Determining standard deviation from a peak Std dev = τW = 4 τ

9. Basics of Chromatography Capacity Factor Selectivity Factor

10. Basics of Chromatography The Van Deemter Equation H = A + B/u + Cu

12. Multiple Flow Paths Tortuosity

13. Components of the VanDeemter

14. Van Deemter Plot

15. How Plate Height is effected by particle size in solid packing

16. Basics of Chromatography Column Efficiency

17. Resolution

18. Basics of Chromatography Resolution

19. The Effect of Mobile Phase on Retention

20. More on Resolution