1. Lecture 18 High Preformance Liquid Chromatography I

2. Column Injector Detector Chromatograph Data Processing

4. Zone broadening: The van Deemter equation H - plate height u - flow rate

6. Stationary phase in HPLC Chemically inert Non-soluble in any imaginable mobile phase Thermal and chemical stability Appropriate physical sorption of analyte Shape: Uniform spherical particles

8. 150 m 2 /g 300 m 2 /g

10. Polar phase Nonpolar phase

17. Percentage of solvents having the same eluent strength

18. B - acetonitrile CH 3 CN

19. Width W is proportional to retention time t r ‘general chromatography problem’

21. Solution of GNP: gradient