1. Use lenses to direct light in order to enlarge images
Light Microscopes
Use lenses to direct light in order to enlarge images

2. Simple Microscopes
Have 1 lens.

3. Compound Light Microscope
Has two sets of lenses.
Eyepiece
Objective Lenses

4. Magnification The change in apparent size.4x
10x
Found by multiplying the power of eyepiece lens by the power of the objective lens.

5. Electron Microscopes
Use a magnetic field in a vacuum to direct a beam of electrons
Can magnify up to 1 million times
3 types
Scanning Electron Microscope (SEM)
Shows the 3-D surface of the object
Transmission Electron Microscope (TEM)
Produces a 2-D image
Scanning Tunneling Microscope (STM)
Shows the arrangement of atoms on the surface of the object

6. Parts of a Compound Light Microscope

7. Parts of a Compound Light Microscope

8. Parts of a Compound Light Microscope

9. Parts of a Compound Light Microscope
Be very careful not to touch the light as it can be HOT!

10. Parts of a Compound Light Microscope

11. Parts of a Compound Light Microscope

12. Parts of a Compound Light Microscope

13. Using the Microscope To Carry One hand on arm One hand on base
Don’t tilt

14. Using the Microscope To Set up
Place on table with arm pointed towards you.
Use the coarse adjustment knob to raise the nosepiece all the way up.
Click the low objective lens into place by turning the nosepiece (Do not turn the nosepiece by grasping the objective lenses!)
Adjust the diaphragm until you see a bright circle of light

15. Using the Microscope
To Clean Lenses
Only clean with lens paper!

16. Using the Microscope Preparing a slide: Caution: Glass!
Place a small drop of water on the slide.
Place the “e” in the drop of water.
Hold a cover slip at an angle to one side of the water drop.
Slowly drop the cover slip onto the slide, trying to avoid air bubbles.

17. Using the Microscope To View a Specimen
With the nosepiece raised all the way up, place a slide over the circular hole in the stage.
Secure the slide with the stage clips.
Looking at the side of the microscope, lower the objective lens until it is almost touching the slide using the coarse adjusting knob.

18. Using the Microscope To View a Specimen (cont.)
Looking through the eyepiece, slowly raise the objective lens. Never lower the objective lens while looking through the eyepiece!
When you can see the specimen, use the fine adjustment knob to bring the image into sharp focus.
When moving to a higher power, always look to the side to make sure lens doesn’t hit the slide.

19. To Use a Microscope To put away the microscope
Raise the objective lens all the way up before removing slide.
Place the slide on your table.
Loosely wrap the cord around the base of the microscope.
Be very careful not to touch the light as it is HOT!
Cover with a dustcover.

20. How to Use a Microscope To put away the microscope (cont)
Carry properly to cupboards.
Never put the microscope on its side.
Clean the slide and cover slip with water...be careful not to drop as they are glass and will cut fingers!
Place the slide cover slip on the paper at the front of the room to dry.