1. SYNBIOLOGY - An Analysis of Synthetic Biology Research in Europe and North America 4,5/4,5 CM SYNBIOLOGY: An Analysis of Synthetic Biology Research in Europe and North America Project Database 30th May 2006 European Commission, Brussels

2. SYNBIOLOGY Brussels, 30 May 2006 General Data Base - Introduction Project Database - -Synbiology project includes development of database of synthetic biology research - -Accessable on Synbiology project website http://www.synthetic-biology.info/ - -Fully indexed and searchable - -Contains references to 700 Synthetic Biology approved and validated according articles according to the classification scheme developed during this project

3. SYNBIOLOGY Brussels, 30 May 2006 Structure of Database Project Database - -Following structure developed in Analysis of Synthetic Biology Literature and Statistical Review - -Theoretical bio-engineering - in silico - -Biological inspired nanotechnology - in vitro - -Practical engineering - in vivo - -Applications of Synthetic Biology (related and enabling technologies) - -Products

4. SYNBIOLOGY Brussels, 30 May 2006 Structure of Database Project Database - -Theoretical bio-engineering in silico - -Biological inspired nanotechnology in vitro - -Practical engineering in vivo - -Applications of Synthetic Biology (related and enabling technologies) - -Products - -Financing/ financial strategy - -Concepts and political strategy - -Modeling of systems based on natural genetic parts - -Genome, Proteome, Metabolone analysis - -Analysis and modelling of molecular networks - -Metabolic profiling - -Modeling of synthetic systems based on artificial genetic parts - -Design principles of systems and networks - -Noise in systems and components - -Information processing and control theory

5. SYNBIOLOGY Brussels, 30 May 2006 Structure of Database Project Database - -Biomimetics, Nanobionics, Evolutionary Nanotechnology - -Computation using biological components and principles - -Selfassembling, biomimetic, biomaterials, bioelectronics - -Single molecule manipulation and/or measurement methods - -Reporters and sensors - -Molecular machines, actuators and device - -Artificial Life - -Theoretical bio-engineering in silico - -Biological inspired nanotechnology in vitro - -Practical engineering in vivo - -Applications of Synthetic Biology (related and enabling technologies) - -Products - -Financing/ financial strategy - -Concepts and political strategy

6. SYNBIOLOGY Brussels, 30 May 2006 Structure of Database Project Database - -Engineering of structural function - -Molecular engineering (rational design) - -Artificial evolution and evolutionary optimization strategies (irrational design) - -Semisynthetic design (rational + irrational design) - -Engineering of regulatory function - -Biochemical or genetic network design and Riboswitches - -Fabrication, characterization, assembly - -The BioBricks strategy and Programmable organisms or systems - -Theoretical bio-engineering in silico - -Biological inspired nanotechnology in vitro - -Practical engineering in vivo - -Applications of Synthetic Biology (related and enabling technologies) - -Products - -Financing/ financial strategy - -Concepts and political strategy

7. SYNBIOLOGY Brussels, 30 May 2006 Structure of Database Project Database - -Molecular Biology Methodology – very exciting improvements - -Analytics – Cellular systems - -Computing - -Biological chemicals - -Biopharmaceuticals - -Biomaterials - -Cell Technology - -Tissue and Organ Engineering, Transplantation - -Reproduction Technologies, In Vitro Fertilisation - -Transgenic Plants and Animals - -Bio-energy Sources - -Theoretical bio-engineering in silico - -Biological inspired nanotechnology in vitro - -Practical engineering in vivo - -Applications of Synthetic Biology (related and enabling technologies) - -Products - -Financing/ financial strategy - -Concepts and political strategy

8. SYNBIOLOGY Brussels, 30 May 2006 Structure of Database Project Database - -Small Molecule Drugs, Bio-Drugs, Bio-Agents (Cells) - -Stem Cells, Cell culture - -Tissues, Organs, Transgenic Organisms - -Flavors, Fragrances, Colors - Pigments, Commodities, Fine Chemicals - -Biopolymers, Biocomposites, Biomaterials, Biomineralisation - -Biomimetics, Bio-Computers - -BioEnergy : Bio-fuels, -gas, - electricity - -Theoretical bio-engineering in silico - -Biological inspired nanotechnology in vitro - -Practical engineering in vivo - -Applications of Synthetic Biology (related and enabling technologies) - -Products - -Financing/ financial strategy - -Concepts and political strategy

9. SYNBIOLOGY Brussels, 30 May 2006 Project Database Development of Database - -16 MIO papers in total were first downloaded - -Selection of Life Science Relevant Subjects according to several overlapping journal indices (e.g. Thomson Index) resulting in 1.5 MIO papers after 1990 - -According to pre-selected Synthetic Biology: key papers and reviews were downloaded - -Other papers cited by these key papers were downloaded - -Papers from „high level expert group“ cited authors were downloaded - -Resulted in 1,100 papers of which 400 were discarded by content gaining in preliminary 700 which are included in the database

10. SYNBIOLOGY Brussels, 30 May 2006 Project Database

11. SYNBIOLOGY Brussels, 30 May 2006 Synthetic Biology Introduction & Structure L1 1-5 L2 1-m L3 1-n Reviews & Concepts Higher Level Basic Science Basic Level Basic Science General & Applied Science Commercialisation, Enabelling Technologies BiotechCompanies Application Primary Literature Secondary Literature ContentView Text in Prose plus Links Impact Factor Citation Index Content Structure Science in General Centers of Excellence Data Base Structured Total Overview Publication commentarial same structure as DataBase ~500 Highest Level Basic Science ~80 > 1000 Project Database

12. SYNBIOLOGY Brussels, 30 May 2006 Project Database - -Sorting Citation Index - -Year - Sorting Citation Index - -Title - Alpha-numerical - -Country, State - -Journal - -First Author - Last Author - -Glossary - for sorting according to frequent technical terms Database Use - Sorting -

13. SYNBIOLOGY Brussels, 30 May 2006 Project Database Database Use - Statistical Review - - -Geographical Distribution - -Time Scale (e.g. # Pub/ SB Class/ Year) - -Frequency of Authors - -Papers per Author