1. Presented by Qiong Cheng in cs6640 Instructor: Prof. Irene T. Weber Prof. Robert W. Harrison Identify an Obesity Association INSIG2

2. Outline Introduction Analysis Methods Analysis Results Conclusion

3. Introduction Obesity - an excess of body fat - a known risk factor for chronic diseases - a kind of metabolic disease INSIG Family and INSIG2

4. INSIG Family ’ s Role

5. INSIG2 is an attractive candidate protein which product inhibits the synthesis of fatty acid and cholesterol [1].

6. Analysis Methods NCBI gene/protein database searching Blast Alignment searching in PDB database PDB database searching LALIGN for pairwise alignment ClustalW for multiple alignment Newho + preammp + AMMP structure alignment tools Rasmol to show protein structure

7. Analysis Results NCBI-Result INSIG2 sequence 1 maegetespg pkkcgpyiss vtsqsvnlmi rgvvlffigv flalvlnllq iqrnvtlfpp 61 dviasifssa wwvppccgta savigllypc idrhlgephk fkrewssvmr cvavfvginh 121 asakvdfdnn iqlsltlaal siglwwtfdr srsgfglgvg iaflatvvtq llvyngvyqy 181 tspdflyvrs wlpciffagg itmgnigrql amyeckviae kshqe //

8. Analysis Results BLAST-Result

9. Alignment of INSIG2 and 1R08 in LALIGN Alignment of INSIG2 and 1RHI in LALIGN

10. Alignment of INSIG2, 1R08 and 1RHI in ClustalW Alignment Tree Generated in ClustalW

11. Analysis Results AMMP-Predicted INSIG2 Molecule Display--- Ribbons Display

12. Analysis Results AMMP-Predicted INSIG2 Molecule Display--- Ribbons Display

13. Analysis Results Comparison-1 in RasMol Predicted INSIG2 --- 1R08 in 3 rd chain

14. Analysis Results Comparison-2 in RasMol The predicted INSIG2 is the red one; 1R08 in 3 rd Chain is the green one;

15. Analysis Results Comparison-2 in RasMol The predicted INSIG2 is the red one; 1R08 in 3 rd Chain is the green one;

16. Analysis Results The longest gap Seek motif SAK Seek the first residue after the largest gap Identify the gap [in RasMol Tool ]

17. Conclusion Study research topics and tools in bioinformatics; Identify INSIG2 structure; The interesting thing is macromolecule structure homology; Further experiments and identification are needed; References: Alan Herbert, Norman P.Gerry A Common Genetic Variant is Associated with Adult and Childhood Obesity Science, Vol312 (2006). …

18. Analysis Results Sequence Alignment Alignment of INSIG2 and 3 rd chain of 1R08 (HRV14)