1. ESHRE 26th Annual Meeting,Rome.
Wednesday 30 June 2010
ESHRE Members’ information session 
New task force - Fertility and viral disease
Enrico Semprini (Italy)

2. New: Task Force – Fertility and Viral Diseases
Coordinator: Augusto E. Semprini
Aim: This Task Force aims to offer a platform of collaboration between ESHRE and the "Centres for Reproductive Assistance Techniques in HIV in Europe" (CREATHE), which is a non-profit association committed to improving the quality and availability of safer conception options to couples who are affected by HIV and/or other transmissible infections and who wish to become parents.
These couples are nowadays subject to discrimination regarding their basic rights for parenthood and for having a family, due to the fear of transmitting the infection to the healthy partner while attempting at conception.
CREAThE O.N.L.U.S. is an NGO registered under Italian law .(
CREAThE is an open network welcoming the inclusion of all figures involved in the field of reproductive care to couples with HIV. To 2

3. Task Force – Fertility and Viral Diseases
The key objectives of CREATHE are:
To promote the availability of services of assisted reproduction to couples affected by HIV or by other sexually transmitted infections.
To promote the offer of services of specific sperm washing to clinical centres unequipped in carrying out this method who intend to offer reproductive assistance to couples affected by HIV or by other sexually transmitted infections.
To spread knowledge and experience regarding the method of sperm washing and regarding reproductive assistance to couples affected by HIV or by other sexually transmitted infections, to physicians and biologists who intend applying this method.

4. Reproductive Centres
ITALY Studio Semprini, Milano Centro di medicina preventiva, Verona
NETHERLANDS Academisch Medisch Centrum, Amsterdam
SPAIN Hospital Galdakao-Usansolo HU Virgen de las Nieves, Granada
SOUTH AFRICA University hospital, Pretoria
SWITZERLAND Kantonsspital St. Gallen, St. Gallen Clinique La Source, Lausanne
U.K.  Chelsea&Westminster hospital, London 
BELGIUM Saint-Pierre hospital, Bruxelles Erasme hospital, Bruxelles
DENMARK Rigshospitale, Copenhagen FRANCE Hospital Paule de Viguier, Toulouse CMCO-SIHCUS, Schiltigheim CHU de Lyon Hòpital Cochin, Paris Hòpital Pitié-Salpètrière, Paris CHU de Rennes
GERMANY Fertility Center, Berlin 
ISRAEL Rambam hospital, Haifa

5. Where do we come from?
Insemination of HIV-negative women with processed semen of HIV-positive partners. (AE Semprini -Lancet. 1992)
NATURAL CONCEPTION IN HIV-NEGATIVE WOMEN WITH HIV-INFECTED PARTNERS (L Mandelbrot Lancet 1997)
QUANTIFICATION OF HIV IN SEMEN: CORRELATION WITH ANTIVIRAL TREATMENT AND IMMUNE STATUS (PL Vernazza- AIDS 1997)
REPRODUCTIVE COUNSELLING FOR HIV-DISCORDANT COUPLES (AE Semprini- Lancet 1997)

6. HIV in semen: sperm washing
Percoll Gradient
45%
90%
NSCs
Motil spermatozoa
HIV Infection
yes No

7. Semen preparation: sperm washing
45%
90%
Gradient
„swim-up“
Insem.

8. Publications
Safety and efficacy of sperm washing in HIV-1-serodiscordant couples where the male is infected:results from the European CREAThE network. Bujan L, Hollander L et al. for the CREAThE network. AIDS 21:1909–1914, Establishing the safety profile of sperm washing followed by ART for the treatment of HIV discordant couples wishing to conceive. A E Semprini , L Bujan et al. Hum Reprod Jul HIV-discordant couples and parenthood: how are we dealing with the risk of transmission? Vernazza PL; Hollander L et al. AIDS 20(4): 635, Multicenter Quality Control of the Detection of HIV-1 Genome in Semen Before  Medically Assisted Procreation. Pasquier C, Anderson D et al. and The CREAThE Network. J Med Virol 78: , 2006

9. Projects
Retrsospective evaluation of the safety and efficacy of semen washing in HIV discordant couples
Retropsective evaluation of the outcome of infertility treatment in HIV positive women
SHARE – Studying HIV And Reproduction in Europe – European registry of reproductive assistance to couples with HIV
Multicenter Quality Control of the Detection of HIV-1 Genome in Semen Before Medically Assisted Procreation

10. Previous Collaboration with CDC
CONRAD's Mission
CONRAD's overall goal is to improve reproductive health, especially in developing countries.  Our main objective is to help develop safe, acceptable, affordable products and methods that provide contraception and/or prevent the sexual transmission of HIV/AIDS and other infections
Manuscript submitted for publication

11. Task Force – Fertility and Viral Diseases
ESHRE- CREAThE Ongoing projects
Web survey on the “Management of HIV-patients requesting reproductive assistance”
SHARE protocol and database
Pre Congress Course in Stockolm 2011 “Assisted Reproduction in couple with HIV”
Campus for healthcare professionals “ Viral Infections in Reproductive medicine “ Berlin 2012
Position Papers