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Mesenchymal Stem Cell Concentration and Bone Repair: Potential Pitfalls from Bench to Bedside by Anna V. Cuomo, Mandeep Virk, Frank Petrigliano, Elise.

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Presentation on theme: "Mesenchymal Stem Cell Concentration and Bone Repair: Potential Pitfalls from Bench to Bedside by Anna V. Cuomo, Mandeep Virk, Frank Petrigliano, Elise."— Presentation transcript:

1 Mesenchymal Stem Cell Concentration and Bone Repair: Potential Pitfalls from Bench to Bedside by Anna V. Cuomo, Mandeep Virk, Frank Petrigliano, Elise F. Morgan, and Jay R. Lieberman J Bone Joint Surg Am Volume 91(5):1073-1083 May 1, 2009 ©2009 by The Journal of Bone and Joint Surgery, Inc.

2 CFU-Fs were counted to estimate the mesenchymal stem cell concentrations. Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073- 1083 ©2009 by The Journal of Bone and Joint Surgery, Inc.

3 The nucleated cell count of the bone marrow aspirate (BMA) was closely correlated to the mesenchymal stem cell (MSC) concentration of the bone marrow aspirate. Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073- 1083 ©2009 by The Journal of Bone and Joint Surgery, Inc.

4 Radiographs of femoral defects in Groups I through VI at twelve weeks. Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073- 1083 ©2009 by The Journal of Bone and Joint Surgery, Inc.

5 Radiographic scores were determined by the percentage of bridging bone at the defect site (1 = 0% to 25%, 2 = 26% to 50%, 3 = 51% to 75%, 4 = 76% to 99% or unicortical bridging of the defect, and 5 = 100% or bicortical bridging of the defect). Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073- 1083 ©2009 by The Journal of Bone and Joint Surgery, Inc.

6 Micro-computed tomography scans showing the bone formation within the femoral defects in Groups III, IV, and V at twelve weeks. Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073- 1083 ©2009 by The Journal of Bone and Joint Surgery, Inc.

7 Histologic cross sections at the midpoint of the femoral defect. Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073- 1083 ©2009 by The Journal of Bone and Joint Surgery, Inc.


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