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B. MALDI IMS Ischemic Hemisphere A.MALDI IMS Control Hemisphere m/z 760.6 Figure 1 300.

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Presentation on theme: "B. MALDI IMS Ischemic Hemisphere A.MALDI IMS Control Hemisphere m/z 760.6 Figure 1 300."— Presentation transcript:

1 B. MALDI IMS Ischemic Hemisphere A.MALDI IMS Control Hemisphere m/z 760.6 Figure 1 300

2 130 4005006007008009001000 1500 8x 170 548.5 760.6 798.6 782.6 Figure 2 548.5 A. Total ions (Control Hippocampus) B. Ischemic Hippocampus Signal Intensity m/z m/z 760.6

3 1500 B. MALDI IMS Ischemic Hemisphere A.MALDI IMS Control Hemisphere m/z 548.5 Figure 3

4 Figure 4 A.TTC Stained Rat Brain Slice 550 B. 16:0/18:1 PC [M+H] + m/z 760.6 1400 C. 16:0/18:1 PC [M+Na] + m/z 782.6 2300 D. 16:0/18:1 PC [M+K] + m/z 798.6

5 200 100200300400500600 m/z 100 Relative Intensity 264.3 548.5 530.5 308.3 252.3 282.3 B. CID (pos ion) m/z 548.5 A. MALDI IMS of Rat Brain 96 hours post TBI m/z 548.5 Figure 5

6 Supplemental Figure 1 100200300400500600 m/z 264.3 524.5 506.6 282.3 252.3 494.5 CID m/z 524.5 Cer 18:1/ 13 C 4 -16:0 [M+H] + -H 2 O Relative Intensity 100 [Cer(d18:1/ 13 C 4 -16:0)-H 2 O] + 400600800 m/z 782.6 760.6 577.5 723.5 524.5 564.5 ESI/MS POPC-H + POPC-Na + Cer(d18:1/ 13 C-16)-H 2 O + Cer(d18:1/ 13 C-16) +

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