1. Topic 2: Cells

2. IB Objectives 2.1 Cell Theory 2.1.1 –Outline the cell theory
2.1.2-Discuss the evidence for the cell theory
2.1.3-State the unicellular organisms carry out all the functions of life
2.1.4-Compare the relative sizes of molecules, cell membrane thickness, viruses, bacteria, organelles and cells, using the appropriate SI units
2.1.5-Calculate the linear magnification of drawings and the actual size of specimens in images of known magnification
2.1.6-Explain the importance of the surface area to volume ratio as a factor limiting cell size
2.1.7-State the multicellular organisms show emergent properties
2.1.8-Explain that cells in multicellular organisms differentiate to carry out specialized functions by expressing some of their genes but not other
2.1.9-State that stem cells retain the capacity to divide and have the ability to differentiate along different pathways
Outline one therapeutic use of stem cells

3. Cell Theory
Many scientists have contributed in developing the three main principles of this theory
All organisms are composed of one or more cells
Cells are the smallest units of life
All cells come from pre-existing cells

4. Evidence to back up #1 Robert Hooke: 1665
First described cells by observing cork with a microscope built by himself
Antonie van Leeuwenhoek: 1668
First observed the living cells and referred to them as “animalcules” (little animals)
Mathias Schleiden: 1838
Stated that plants are made of “independent, separate beings” called cells
Theodor Schwann: 1839
Made a similar statement as Schleiden about animals

5. Evidence to backup #2
The second principle continues to gain support today, as we have not been able to find any living entity that is not made of at least one cell

6. Evidence to backup #3 Louis Pasteur: 1860
He sterilized chicken broth by boiling to show that living organisms would not “spontaneously” reappear.
Only after exposing the broth to preexisting cells was life able to re-establish itself in the broth

7. Functions of Life Metabolism Growth Reproduction Response Homeostasis
Nutrition

8. Functions of Life
All these functions are tied together to produce a functioning living unit
Metabolism includes all the chemical reactions that occur within an organism
Growth may be limited but is always evident in one way or another
Reproduction involves heredity molecules that can be passed to offspring
Homeostasis refers to maintaining a constant internal environment.
Nutrition is all about providing a source of compounds with many chemical bonds which can be broken to provide the organism with the energy and the nutrients necessary to maintain life

9. Cells and Sizes
Cells are made up of a, but all are microscopically number of different subunits
These subunits are often of a particular size, but all are microscopically small.
In most cases, microscopes with high magnification and resolution are needed to observe cells and especially their subunits
Resolution refers to the clarity of a viewed objects

10. Cells and Sizes
Light microscope-uses light, which passes through the living or dead specimens, to form an image.
Stains may used to improve viewing of parts
Electron microscope-provides us with the greatest magnification (over X) and resolution.
These use electrons passing through a specimen to form an image

11. Cells and Sizes Level of Organization and size
Cells (most cells are up to 100 µm)
Organelles (most up to 10 µm)
Bacteria (most up to 1µm)
Viruses (most are 100 nm)
Membranes (most up to 10 nm)
Molecules (most up to 1 nm)

12. Cells and Sizes
If you want to calculate the actual size of a specimen seen with a microscope, you need to know the diameter of the microscope’s field of vision
This may be calculated with a special micrometer or with a simple ruler on a light microscope.
The size of specimen can then be calculated in the field

13. Cells and Sizes
The size of the specimen can then be calculated in the field
Drawing or photographs are often enlarged.
To calculate the magnification, you need this formula:
magnification = size of image divided by size of specimen
Scale bars are often used with a micrograph or drawing so that actual size can be determined

14. Calculating linear magnification of drawings or photos
A scale bar is a short line, usually drawn on an electron micrograph, to allow you to determine the actual magnification of the photograph

15. Calculating magnification
1. measure scale bar carefully with a metric ruler, in mm.
2. convert the mm into the same units as the scale bar (μm or nm)
3. divide this by the number given on the scale bar (μm or nm)

16. Converting metric units
1 nm
nanometer
1 μm
micrometer
1 mm
millimeter
1 m
meter m m
.001m 1m

17. Example: 15 mm = 15000 μm (change to same units as microscope photo)
Magnification = 3000x
Bar = 5 μm
(measure bar with a metric ruler = 15 mm)

18. If the magnification is stated, you can calculate the actual size
1. measure the diameter of the cell
2. divide by the magnification
3. convert to a commonly used unit (nm or μm)

19. Example: Magnification = 750x
41 ÷ 750 = mm
0.055 mm = 55 μm
Diameter of the cell is 55 μm
Diameter of cell is 41 mm (measure with metric ruler)

20. Practice: p. 96, a and b (cilia) p. 98 b (bacterium)
p. 105 fig 6.12 (ER)

21. Practice answers:
p. 96, a and b (cilia)
7mm = 1 μm = 7000x magnification
(convert mm into μm)
p. 98 b (bacterium)
19mm = μm (conversion)
19000/0.5 = 38000x magnification

22. p. 105 fig 6.12 (ER)
9mm: convert to nm = 9,000,000
Divided by 200 = 45000x

24. Limiting Cell Size The surface to volume ratio
In the cell, the rate of heat and waste production and rate of resource consumption are functions of (depend on) its volume
Most of the chemical reactions occur in the interior of the cell and its size affects the rate of thee reactions
The surface of the cell, the membrane, controls what materials move in and out of the cell
Cells with more surface area per unit volume are able to move materials in and out of the cell, for each unit volume of the cell

25. Limiting Cell Size
As the width of an object such as a cell increases, the surface area also increases but at a much slower rate than the volume
This is shown by the following table in which you can see that the volume increases by a factor calculated by cubing the radius; at the same time, the surface increases by a factor calculated by squaring the radius
Cell radius (r)
0.25 units
0.5 units
1.25 units
Surface area
0.79 units
3.14 units
7.07 units
Volume
0.06 units
0.52 units
1.77 units
Surface area:volume
13.17:1
6.04:1
3.99:1

26. Limiting Cell Size
This means that a large cell has relatively less surface area to bring in needed materials and to rid the cell of waste, than a small cell.
Because of this, cells are limited as to the size they can attain and still be able to carry out the functions of life.
Sphere formulas:
Surface area = (four)(pi)(radius squared) = 4πr2
Volume = (four-thirds)(pi)(radius cubed) = 4/3πr3

27. Limiting Cell Size
This means large animals do not have larger cells they just have more cells
Cells that are larger in size have modifications that allow them to function efficiently
Shape changes-such as spherical to long and thin
Infolding and outfolding

28. Cell Reproduction and differentiation
One of the functions that many cells retain is the ability to reproduce themselves.
In multicellular organisms, this allows the possibility of growth.
It also allows for the replacement of damaged or dead cells
Multicellular organisms like ourselves usually start out as a single cell after some type of sexual reproduction

29. Cell Reproduction and differentiation
Multicellular organisms like ourselves usually start out as a single cell after some type of sexual reproduction
This single cell has the ability to reproduce at a very rapid rate, and the resulting cells then go through a differentiation process to produce all the required cell types that are necessary for the well-being of the organism
The number of different cell types from the one original cell may be staggering.
This differentiation process is the result of the expression of certain genes but not others. Genes, segments of DNA on a chromosome, allow for the production of all the different cells in the organisms.
Therefore, each cell contains all genetic information for the production of the complete organism. However, each cell becomes a specific type of cell dependent on which DNA segment become active.

30. Cell Reproduction and differentiation
Some cells have a greatly, or even completely diminished ability to reproduce once they become specialized
Examples is muscle cells and nerve cells

31. Stem Cells
There is a population of cells within organisms that retain their ability to divide and differentiate into various cell types—Stem cells
Plant cells—found inside the meristematic tissue- near root and stem tips
Gardeners-take cuttings of this area and use them to produce new plants
Pluripotent or embryonic stem cells—cells in mammals which retain the ability to form any type of cell in an organism and can even form a complete organism

32. Stem Cells
When stem cells divide to form a specific type of tissue, they also produce some cells that remain as stem cells—this allows for the continual production of a particular type of tissue.
Possibility to treat certain human diseases
But a problem discovered early in research was that stem cells cannot be distinguished by their appearance only by their behavior.

33. Stem Cell Research and Treatments
Grow embryonic stem cells in culture so they can be used to differentiate cells lost due to injury and disease—This involves therapeutic cloning.
Parkinson’s disease and Alzheimer's disease— caused by a loss in brain cells
Certain forms of diabetes-depleted cells in pancreas
Still Experimental

34. Stem Cell Research and Treatments
Type of currently successful treatments
There is also tissue specific stem cells—these reside in certain tissue types and can only produces new cells of that particular tissue.
Example-blood stem cells—replace the damage bone marrow of some leukemia patients

35. Stem Cell Research and Treatments
There are important ethical issues involved in stem cell research. Especially controversial is the use of embryonic or pluripotent stem cells.
This is because these cells come from embryos often obtained from laboratories carrying out in-vitro fertilization (IVF).
To gather cells invloves death of the embryos and opponents argue that this represents the taking of a human life.
On the other hand, it is argued this research could result in the significant reduction of human suffering and is, therefore, totally acceptable