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Fig. S1 B A GLC82 A549 Calu3 H460 H1299 EGFR p-EGFR MEK 1/2 p-MEK 1/2
AKT pAKT RAS-Act RAS-T actina LS174T GEO HCT15 HCT116 SW480 SW620 EGFR p-EGFR MEK 1/2 p-MEK 1/2 AKT pAKT RAS-Act RAS-T actina
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Table S.1A Mutation Status of Cell Lines Used in This Study
GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE p.G12D c.35G>A LS174T p.G12A c.35G>C GEO p.G12V c.35G>T SW480;SW620 p.G13D c.38G>A HCT116; HCT15 p.G13A c.38G>C p.G13V c.38G>T p.G13G c.39C>G c.39C>A c.39C>T p.G12S c.34G>A A549 p.G12R c.34G>C p.G12C c.34G>T p.A59T c.175G>A p.Q61K c.181C>A p.Q61E c.181C>T c.181C>G p.Q61P c.182A>C KRAS
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KRAS GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE p.Q61R
c.182A>G p.Q61L c.182A>T p.Q61H c.183A>C H460 KRAS
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Table S.1B Mutation Status of Cell Lines Used in This Study
GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE p.Q61P c.182A>C p.Q61L c.182A>T p.Q61R c.182A>G p.Q61H c.183A>C p.Q61Q c.183A>G c.183A>T p.G61K c.181C>A H1299 p.G61E c.181C>G p.G13S c.37G>A p.G13R c.37G>C p.G13C c.37G>T p.G12R c.34G>C p.G12S c.34G>A NRAS
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Table S.1C Mutation Status of Cell Lines Used in This Study
GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE p.K601E c.1801A>G p.V600E c.1799T>A p.D594G c.1781A>G BRAF
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Table S.1D Mutation Status of Cell Lines Used in This Study
GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION EXON CELL LINE p.H1047R c.3140A>G exon 20 HCT116; GLC82; LS174T p.H1047L c.3140A>T p.P539R c.1616C>G exon 9 p.Q546K c.1636C>A A549 p.Q546E c.1636C>G p.E81K c.241G>A exon 1 p.R88Q c.263G>A p.G106V c.317G>T p.N345K c.1035T>A exon 4 p.R93W c.277C>T p.S158L c.473C>T exon 2 p.H160N c.478C>A p.R38H c.113G>A p.E542K c.1624G>A p.E542Q c.1624G>C p.E545K c.1633G>A H460;HCT15 p.E545Q c.1633G>C PIK3CA
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PIK3CA GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION EXON CELL LINE
p.G118D c.353G>A exon 2 p.G12D c.35G>A exon 1 p.H1047Y c.3139C>T exon 20 p.C420R c.1258T>C exon 7 p.H701P c.2102A>C exon 13 p.K184E c.550A>C exon 2 p.C901F c.2702G>T exon 18 p.M1004I c.3012G>T p.G1007R c.3019G>C p.G1049R c.3145G>C p.R108H c.323G>A exon 1 PIK3CA
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Table S.1E Mutation Status of Cell Lines Used in This Study
GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE p.R248W c.742C>T p.Y220C c.659A>G P53 GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE p.F241S c.722T>C p.Y65C c.194A>G p.E150Q c.448G>C p.K62R c.185A>G p.K267fs*9 c.800delA PTEN GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE p.R154W c.460C>T p.E221 c.661G>T p.S251N c.752G>A p.I295fs*23 c.882_882delG MEK 1/2
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Table S.1F Mutation Status of Cell Lines Used in This Study
GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE AKT p.A377V c.1130C>T GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE EGFR p.E746_A570 c.2236_2250del15
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8 5 6 1 Fig. S2 KRAS mutation KRAS (G12and G13) mutation
PIK3Ca mutation NRAS mutation NO YES RE 1 4 SE 2 Χ-test p = RE SE No mut 1 2 G12 3 G13 Χ-test p = NO YES RE 2 5 SE 3 1 Χ-test p = NO YES RE 7 SE 3 1 Χ-test p = 1 0.8466 0.1939 0.7273 8 1 6 5
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Fig. S7 Fig. S3 H460 A549 LS174T HCT15 GLC82 CALU3 HCT116 H1299 SW620
GEO Fig. S3
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Fig. S4 RTK GPCR P cAMP RA signaling ADCY7 β γ αs Grb2 SOS Shc ATP Ras
PKA R AKAP13 Raf-1 PKA C MEK1/2 RA signaling NCOA3 TAF3 MAPK NR1H2 RXRA Cell survival Cell proliferatiom Cell proliferation Cell differentiation
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