1. The Cell Membrane

2. What is the cell membrane? AKA: Plasma membrane AKA: Plasma membrane The boundary between the cell and the environment The boundary between the cell and the environment Does every cell have a cell membrane? 1. Yes, Each and every cell has a cell membrane.

3. Cell membranes help maintain homeostasis, what is that? 1. Cells breathing 2. Cells getting blood to them 3. Cells maintaining internal conditions 4. Cells reproducing 12345678910111213141516171819202122232425262728

4. The cell membrane allows nutrients to come into the cell 1. Cell membranes help to maintain homeostasis 1. Examples: Controls amounts of glucose, amino acids, lipids

5. Cell Membranes are… Fluid Mosaic Selectively Permeable Phospholipid Bilayers Fluid Mosaic Selectively Permeable Phospholipid Bilayers Huh??? Huh??? Let’s break it down… Let’s break it down…

6. Selectively Permeable… What does selective mean? What does selective mean? What does Permeable mean? What does Permeable mean?

7. Cell membranes are SELECTIVELY PERMEABLE… -the cell membrane lets some molecules in and keeps others out

8. Phospholipid Bilayer… Phospholipids make it up Phospholipids make it up It has TWO (bi) LAYERS It has TWO (bi) LAYERS Just as a bicycle has two tires…the bilayer has two layers Just as a bicycle has two tires…the bilayer has two layers

9. Structure of the Cell Membrane What does the cell membrane look like up close? What does the cell membrane look like up close? Notice the Notice the 2 layers! 2 layers! Lets look at one of these structures up close

10. Structure of Cell Membrane A.Phospholipids 1. Phosphate head a. Polar b. Hydrophilic c. Make up the outer borders of the membrane

11. 2 Fatty acid tails (lipids=fats, oils, etc) 2 Fatty acid tails (lipids=fats, oils, etc) a. Nonpolar b. Hydrophobic c. Make up the inner part of the membrane

12. Structure of Phospholipid

13. The phospholipids arrange Themselves in a very specific way

14. The key is…Polar vs. Nonpolar Polar: positive and negative ends (b/c electrons are not shared equally) 1. Example: Water Nonpolar: does not have oppositely charged ends (atoms share electrons equally) Which of the above is hydrophilic? Hydrophobic? Which of the above is hydrophilic? Hydrophobic?

15. So…Why are the phospholipids arranged tail to tail? a. Water is inside and outside the cell b. Phosphate group is hydrophilic (polar) end 1. Attracts water c. Fatty acid tail end is hydrophobic (nonpolar) 1. Repels water

16. Thus we get the bilayer of phospholipids… 2 layers of phospholipids make up a cell membrane Remember polar heads and nonpolar tails

17. Arrangement of phospholipids “tail to tail” due to water inside & outside the cell

18. Arrangement of cell surface proteins

19. Cell Membrane Structure

20. Let’s Begin to Build Our Membrane! 1. Take 1 bag with materials for you & your partner 2. You are going to build your phospholipids first a. Marshmallows represent your phosphate head b. Toothpicks represent your fatty acid tails 1. Break these in half. Please include unsaturated fatty acid tails. c. Make sure to line up the phospholipid bilayer correctly! Think polar & nonpolar!

21. When something is hydrophilic, it… 1. Has a chemical makeup that likes to be around water 2. Has a chemical makeup that does not like to be around water

22. Where would you expect to find water in this cell membrane? 1. Here 2. Here

23. Structure of Cell Membrane (cont.) C.Cholesterol 1. Helps to stabilize the phospholipids and keep them from sticking together

24. Building Time! 1. Now let’s add your cholesterol into the membrane 2. Use the gummy bears that are in your bags to represent the cholesterol a. Be sure to place them correctly b. Check with your teacher once your group has completed this 3. We will stop here for today. Please put your group’s name on the bag and put your phospholipids and cholesterols back in bag

25. Bell Work 1. Please put the parts of the cell membrane that we built yesterday back together a. Phospholipid bilayer b. Cholesterols

26. Structure of the Cell Membrane (cont.) D.Proteins: Help regulate which molecules enter and which molecules leave a cell.

27. 1.Types of proteins in the cell membrane a. Carrier Protein 1. Use energy to help needed substances or waste materials to move through the cell membrane which would otherwise be too big (or polar).

28. Let’s Build It! Take your Pretzel out of the bag. Take your Pretzel out of the bag. This will represent the carrier protein. This will represent the carrier protein. Keeping in mind what we just talked about in class, think of a way you can turn this into what a carrier protein is. Keeping in mind what we just talked about in class, think of a way you can turn this into what a carrier protein is.

29. Types of proteins (cont.) b. Channel or Pore protein 1. Hydrophilic channel – allows lipid insoluble substances to pass in and out of cell. 2. Does NOT REQUIRE ENERGY!!!

30. Let’s Build It! 1. Take the Twizzler out of your bag. 2. This represents the channel protein. 3. You will need to split this in half in order to represent both sides of the channel.

31. Arrangement of cell surface proteins

32. Structure of Cell Membrane (cont.) E.Nonpolar interior zone- true barrier that separates the cell from its surroundings 1. Many polar particles like sugars, proteins, ions, & most cell wastes cannot cross this zone b/c they are repelled by the nonpolar region

33. IV.) Fluid Mosaic Model of Cell Membrane A.Lipid bilayer is not strong & firm like a hard shell, but it is fluid like a soap bubble 1.Often called a fluid mosaic model B.Individual phospholipids, arranged side by side, float within the bilayer 1.Cholesterol prevents phospholipids from sticking together

34. Cell Membrane Structure Overview

35. Honors Only

36. Types of proteins (cont.) c. Glycoproteins 1. Protein with what macromolecule attached to it? Carbohydrate Carbohydrate 2. Functions for cell to cell recognition

37. Let’s Build It! 1. Take the Pretzel Stick out. 2. This will represent the “glyco” part of the glycoprotein. 3. Also take out the large Gum Drop. 4. This will represent the protein part of the glycoprotein

38. Types of proteins (cont.) d. Receptor Protein 1. These have binding sites for molecules such as hormones or substrates to bind to

39. Let’s Build It! Take your Fruit Gum Drop out of the bag. Take your Fruit Gum Drop out of the bag. This will represent your receptor protein. This will represent your receptor protein. You will need to use your fingers to “carve out” the active site for this receptor protein You will need to use your fingers to “carve out” the active site for this receptor protein

40. Types of proteins (cont.) e. Enzymatic Protein 1. Catalyze specific reactions along the inside of the cell membrane

41. Let’s Build It! Take a scrap sheet of paper and cut out a Pac- Man Shape Take a scrap sheet of paper and cut out a Pac- Man Shape Put this cut-out into your membrane Put this cut-out into your membrane Which way would the protein fit into the membrane? Which way would the protein fit into the membrane? What would fit into Pac-Man’s mouth? What would fit into Pac-Man’s mouth?