1. Effect of Environmental Conditions on Heart Rate: Experimenting with Daphnia AP Biology Lab 10

2. http://cfb.unh.edu/cfbkey/html/anatomy.html

3. http://ebiomedia.com/gall/classics/Daphnia/daphnia_behave2.html Daphnia Heart Rate

4. Procedure Obtain 2 concave depression slide Add a Daphnia to one of the slides Top with inverted second slide and secure with rubber bands OR cover slip

5. Heart Rate and Temperature Use culture dishes to set up baths of varying temperatures Start with the coolest bath Count number of heart beats in 10 seconds

6. Heart Rate and Temperature

7. Effect of Temperature on Metabolism Ectothermic animals –Rate of metabolism dependent on temperature –Metabolic rate and activity increases dramatically between 5  and 35  C

8. “Q 10 ” Increase in Metabolism expressed in terms of “Q 10 ” –Rate of metabolic rate at one temperature compared to the metabolic rate at a temperature 10  C lower

9. “Q 10 ” Q 10 =rate at higher temp. rate at lower temp. Q 10 = [k 2 /k 1 ] k 1 = heart rate at lower temp. k 2 = heart rate at higher temp.

10. Drug Effects on Heart Rate Nicotine—stimulant –Increases heart rate and blood pressure Alcohol—depressant –At first lowers blood pressure, but then rebounds to increase blood pressure Caffeine—stimulant –Increases heart rate and blood pressure Aspirin –Decrease heart rate and blood pressure