1. Reduced Tumour RNA Integrity in Response to Chemotherapy in Breast Cancer Patients Northern Health Research Conference May 28, 2010 Sudbury, ON

2. Disclosure Statement Patents related to the findings presented in this seminar have been filed and are entering the national phase in Canada, the U.S.A., Europe, Australia, and Japan A company (RNA Diagnostics, Inc.) will commercialize the intellectual properties associated with the patent, for which I am a shareholder and serve as its Chief Scientific Officer

3. Locally Advanced Breast Cancer A breast cancer that has progressed locally but has not yet spread outside the breast and local lymph nodes Includes large breast tumors (more than 5 centimeters in diameter), those that involve the skin of the breast or the underlying muscles of the chest wall, and cancers that have extensive involvement of the local lymph nodes (in the axilla or in the soft tissues above and below the collarbone) Also includes inflammatory breast cancer, a rapidly growing type of cancer that makes the breast appear red and swollen, without a distinctive breast lump

4. Treatment of Locally Advanced Breast Cancer For most women with locally advanced breast cancer, chemotherapy is the first component of the treatment, before surgery (aka “neoadjuvant chemotherapy”) Typically, patient tumours are treated with an anthracycline (doxorubicin or epirubicin) in concert or followed by a taxane (paclitaxel or docetaxel) In some countries pre-operative chemotherapy has become standard for treatment of most breast cancers, since survival rates appear to be similar if chemotherapy precedes surgery

5. Typical Response Rates for Treatment of Locally Advanced Breast Cancer by Chemotherapy Typically ~30% of patients with locally advanced breast cancer have a clinical complete response to neoadjuvant chemotherapy (complete disappearance of all detectable malignant disease in breast by palpation) In contrast, only ~10% of patients with locally advanced breast cancer have a pathologic complete response to neoadjuvant chemotherapy (complete eradication of disease at the cellular level)

6. Utility of a Biomarker of Response to Chemotherapy The low rates of clinical complete response and pathologic complete response in patients with inflammatory breast cancer indicate that vast majority of these patients will experience disease recurrence and/or progression of disease Thus, it would be highly useful to be able to monitor response to chemotherapy in inflammatory breast cancer patients If tumours show a lack of response to chemotherapy, then chemotherapy (and its side effects) can be discontinued and downstream options such as surgery, radiation therapy, or other chemotherapy drugs could be quickly considered

7. Tumour RNA Quality (Integrity) as a Biomarker of Chemotherapy Response Unlike DNA, RNA is much less stable in normal and tumour cells Recent evidence suggests that chemotherapy agents may induce RNA damage and the production of RNA-degrading enzymes It is possible that a reduction in tumour RNA integrity could serve as a useful biomarker of chemotherapy response in patients with inflammatory breast cancer.

8. The RNA Integrity Number (RIN): A quantitative measure of RNA quality, not quantity

9. NCIC-CTG MA.22 Clinical Trial Patients with locally advanced breast cancer are typically treated with anthracyclines followed by taxanes Popular Regimens:AC→T, EC →T, FEC 100 →T (all q3wX6) However, <40% of patients respond to taxanes after anthracycline-based chemotherapy MA.22 is assessing whether anthracycline/taxane chemotherapy may have greater therapeutic efficacy if the drugs are co-administered rather than given sequentially

11. Dose Level 1 Dose Level 2 Dose Level 3 Dose Level 4 Dose Level 5 Dose Level 6 Dose Level 7 Tumour RIN Values of 50 MA.22 Patients (Pre-, Mid-, And Post-Treatment) (p=0.05) (p=0.12) P-value from one-way Analysis of Variance (ANOVA)

12. Relationship Between Tumour RIN and Tumour Cellularity (Extent)

13. Plot of Tumour RIN Versus Tumour Cellularity (Extent) for Each Patient in the MA.22 Clinical Trial Tumour RIN is NOT a surrogate measure of tumour cellularity

14. Factors AssessedBaseline P-value 1 Mid- treatment P-value 1 Post- treatment P-value 1 Maximum RIN and Dose Level0.610.050.12 Maximum RIN and Best Clinical Response (CR;PR/SD/PD) 0.120.610.64 Maximum RIN and Pathologic Response (CR;PR/SD/PD) 0.960.010.28 Tumor Cellularity and Dose Level0.140.770.78 Tumor Cellularity and Best Clinical Response (CR;PR/SD/PD) 0.860.950.91 Tumor Cellularity and Pathologic Response(CR;PR/SD/PD) 0.720.680.01 RIN and Tumor Cellularity by Response to Clinical Treatment Data suggests that mid-treatment tumour RIN determinations can predict post- treatment pathologic complete response 1 P-value from one-way Analysis of Variance (ANOVA). Pathologist cannot predict whether patient will undergo pathologic complete response by examining tumour cellularity mid-treatment Correlation between low mid-treatment tumour RIN values and drug dose level suggests that tumour RIN may clearly be a biomarker of drug response

15. Future Perspectives Related to the Use of the RIN as a Biomarker of Chemotherapy Response – Part I We have heard that Dr. John Bartlett of the University of Edinburgh in the UK has validated our findings in an independent cohort of breast cancer patients treated with an anthracycline-based chemotherapy regimen Our findings will hopefully be further validated in an additional independent cohort of breast cancer patients treated with various chemotherapy regimens in collaboration with I-Spy Clinical Trial in the U.S.A. The I-SPY trial will also assess how soon after treatment statistically significant reductions in tumour RIN can be observed, which predict pathologic complete response in cancer patients

16. Future Perspectives Related to the Use of the RIN as a Biomarker of Chemotherapy Response – Part II Future studies will also focus on determining whether similar reductions in tumour RIN can be observed in patients with other solid and nonsolid tumours that are being treated with a variety of chemotherapy drug classes A new company (RNA Diagnostics, Inc.), Inc. has negotiated an exclusive license to the IP associated with the patent. It will fund further validation studies and productize what we are calling the “RNA Disruption Assay” or RDA for measuring chemotherapy response in cancer patients.

17. A New Research Grant! The Cancer Research Fund of the Ontario Institute for Cancer Research has awarded a $381,847 grant to further these promising studies Principal Investigator: Dr. A. Parissenti of NOSM/LU/SRH Co-investigators Dr. D. Maclean, graduate student S. Fabris, animal studies Dr. M. Trudeau, Sunnybrook Hospital, MA.22 clinical trial Dr. L. Esserman, UCSF San Francisco, I-SPY clinical trial Drs. L. Shepherd, J. Chapman, NCIC Clinical Trials Group

18. Acknowledgements Judy-Anne W. Chapman 2, Harriette J. Kahn 3, Baoqing Guo 1, Lei Han 2, Patti O’Brien 2, Mark P. Clemons 4, Roberta Jong 3, Rebecca Dent 3, Barbara Fitzgerald 4, Kathleen I. Pritchard 3, Lois E. Shepherd 2, Stacey Santi 1, and Maureen E. Trudeau 3 1 Regional Cancer Program, Sudbury Regional Hospital, Sudbury, ON, 2 National Cancer Institute of Canada Clinical Trials Group, Queen’s University, Kingston, ON, 3 TorontoSunnybrook Health Sciences Centre, Toronto, ON, 4 Princess Margaret Hospital, University Health Network, Toronto, ON