1. H. M.J.Bandara Plant Quarantine Officer Sri Lanka
Biotechnology: Presant Status & biosafety concerns Department of Agriculture
H. M.J.Bandara
Plant Quarantine Officer
Sri Lanka

2. Department of Agriculture
Mandatory Crops: Cereals, legumes, condiments, fruit crops, root & tuber crops, oil seed crops vegetables, floricultural plants
Activities:
Research & development, Seed certification & plant protection, Planting material production Distribution & Plant quarantine, Germplasm management, Botanic gardens, Agriculture extension, Education & training,
Finance & administration

3. Tissue Culture Molecular techniques (Based on nuclear acid & proteins)
Biotechnology
Tissue Culture Molecular techniques (Based on nuclear acid & proteins)
Biotechnology is defined in many ways.
Tissue culture is considered one of the techniques. It’s associated directly with genetic transformation.

4. Banana Tissue Culture Papaya tissue culture
HORTICULTURAL CROP RESEARCH AND DEVELOPMENT INSTITUTE- Tissue Culture Program
Banana Tissue Culture
Papaya tissue culture

5. HORTICULTURE CROP RESEARCH AND DVELOPMENT INSTITUTE
Virus indexing (serological)
Banana
Papaya
Citrus
Microbiology
Food Research Unit
Fermentation Food analysis

6. PLANT VIRUS INDEXING CENTER
Serology and PCR Based Virus Indexing- Banana Virus
Pineapple
RT PCR for Detection of RNA Virus

7. SITHA ELIYA POTATO RESEARCH CENTER: Tissue Culture Program Potato
Straw berry
Pineapple

8. RARDC, Bandarawela Tissue Culture Program Potato Anthurium Gerbera
Strawberry
Madonna lily
Virus indexing of potato by ELISA

9. RARDC, Agunakolapelessa
Banana
Thubakarwial

10. RICE RESEARCH AND DEVELOPMENT INSTITUTE:Rice Biotechnology
Anther Culture
Exploitation of Somaclonal Variation
Wide Hybridization

11. NATIONAL BOTANIC GARDENS-Tissue Culture Program
Propergation of Endemic & rare Plants
Floriculture Plants
Mutation induction

12. PLANT GENETIC RESOURCE CENTER: Tissue Culture Program
Banana
Citrus spp
In vitro pineapple potato Conservation
Papaya tissue Culture

13. PLANT GENETIC RESOURCE CENTER: Tissue Culture Program
Anther Culture in rice Haploid Culture
a good system for transformation

14. PGR/RRDI- Rice research PCR based DNA markers for breeding/gene pyramiding
Gall midge resistance
Thrips resistance
Bactria leaf blight resistance
BPH resistance

15. Genetic Characterization by DNA markers
Mango - Mangifera zeylanica Magifera indica
Maize(PGRC/MI)
Finger printing of hybrids
Pathogens - Ralstonia, BLB
Onion - Characterization & breeding

16. Introgression of genes from wild relatives
Wild tomato X tomato
Wild okra X okra

17. Future Work SSR markers for rice
AFLP for fingerprinting of germplasm & Varieties
Northern blot techniques for
Analysis of HSP regular gene Expression
Isolating the fragment for Coat protection gene papaya Ring spot virus

18. Application of Tools in Biosafety Regular Measures
Tissue Culture Safe handling of materials Restricted multiplication
Serological methods Testing &detection of foreign
Genes bases in proteins
Serological method should not be the sole method.
Genes expression (transgenic)is spatially & temporally controlled
PCR/Blot methods Based on DNA More sensitive method.Detect only presence or foreign DNA

19. National Plant Quarantine Service
Requirements
With the existing infrastructure & function and provided with additional strength (Trained staff, Detection equipment, Risk assessments Studuo) NPQS could undertake the regulatory activity

20. Institutional Biosafety Committee
Liaison with the national committees
Identification of institutes:transformation work,GMO detection /testing,regulation & risk assessment
Preparation of guidelines on biosafety regulations,risk assessments & management
Maintain constant access to relevant data base or registers both local & foreign
Monitoring & evaluation of programs
Building awareness

21. Requirements for Implementation of the Biosafety Procedures
Policy level support
Financial,administrative & legislative support
Biosafety clearing house mechanism
Monitoring, evaluation & inspection services
Training of decision makers,&technical level scientists & technicians
Facility enhancement
Public awareness, education & promotion of public participation including stake holders.

22. GENETIC Transformation of Agricultural Crops
Policy level decisions & if necessary, changes
Identification of priority problems/crops
Identification of Institutes
Facility development

23. THANK YOU