1. NICO 2000 LTD. Introducing the New Range of PC-based Ion-Analysers ELIT 9801 – ELIT 9804 – ELIT 9808 Document No. IAP 05-03-c.ppt compiled by Heinz Kreuzberg, Shree Barve, Ameet Barve & Chris Rundle London, UK - May/June 2003 From NICO 2000 Ltd.

2. NICO 2000 LTD. A Multitude of Applications for Ion Analysers COSMETICS AGRICULTURE WATER MONITORING INDUSTRIAL PRODUCTION WASTE WATER TREATMENT FISHERIES POLLUTION CONTROL BLOOD TESTING

3. NICO 2000 LTD. Contents 1.Introduction to Ion Measurement 2.pH Measurement 3.Product Description 4.Features – Benefits – Advantages 5.Applications 6.Hardware Details 7.Software Details 8.Using the ISE / pH Ion-Analyser 9.Using the 4- & 8- Channel Analysers 10.The NICO 2000 Product Range

4. NICO 2000 LTD. Introduction to Ion Measurement Ion-selective Electrodes (ISEs) allow us to determine the concentration of a specific ion in aqueous (& in rare cases non-aqueous) solutions. An Ion-Analyser or Ion Meter is required to convert the electrical signal from the ISE into a relevant unit of concentration (ppm or mol/litre). The measurement made is not an absolute one but rather a comparison of a sample measurement with a calibration measurement (calibration curve) The calibration procedure uses Ion- Standards (solutions with a precisely defined ion concentration) and specifically diluted calibrating solutions. Usually, 1:10 serial dilutions from a 1000 ppm ion-standard. Measurements with ISEs are temperature sensitive. An exact temperature compensation for measurements taken at different temperatures is not possible. Therefore, calibration and sample measurement should be done within the same temperature range (+/- 1 0 ).

5. NICO 2000 LTD. Typical ISE Calibration Graph NB: X-axis units are the logarithm of the Molar Activity of the Ion

6. NICO 2000 LTD. ISE Measurement with ELIT ISE/pH Ion-Analyser For educational purposes, sample results are plotted on the Calibration Graph

7. NICO 2000 LTD. pH Measurement with ELIT ISE/pH Ion Analyser pH is a measure of the Acidity or Alkalinity of a solution. Actually measures the concentration of Hydrogen Ions. Is based on a logarithmic relationship between the sensor signal (mV) & the Hydrogen ion concentration (mol/L). Calibration standards for pH measurements are called Buffers due to their ability to keep the pH value stable when small amounts of acid or alkaline substance added. The most common Buffers are pH 4, pH 7 & pH 10. Like all Ion-selective measurements, pH measurements are influenced by temperature changes. Therefore, instruments have manual or automatic temperature compensation facilities. To simplify the handling of results of pH measurements, the 14 decades of hydrogen ion concentration are converted into a linear (pH) scale from 0 to 14 using the Soerensen equation: pH value = negative Logarithm of the Hydrogen ion concentration. pH 0 is extremely acidic, pH14 is extremely alkaline & pH 7 is neutral. 2-Channel pH Display “Virtual Instrumentation”

8. NICO 2000 LTD. Typical pH Values

9. NICO 2000 LTD. Product Description 3 Types of Ion-Analyser are available: ELIT 9801 = ISE/pH Ion-Analyser ELIT 9804 = 4-Channel Ion-Analyser ELIT 9808 = 8-Channel Ion-Analyser They are powerful tools for measuring & monitoring Ion Concentrations, pH, Redox (ORP) & Temperature They contain high quality Electrode Amplifiers, High Resolution Analogue- to-Digital converters, a Powerful Microprocessor & a state-of-the-art opto-isolated Serial interface They are primarily PC-based Instruments - but the hardware is also compatible with any computer with a serial port (RS232) They are powered by sophisticated software which runs on any Microsoft Windows Operating System; - Windows 98 or later (2000, ME, XP). Menu-driven software guides the user reliably through the measurement process & supports GLP effectively. They can perform Single Measurements,on a series of samples or Continuous Monitoring of changes in individual solutions. Acquired data is displayed online in Tables and Graphs. Data are stored as ASCII text files which are easily Exportable to other software packages such as Excel, Labview etc.

10. NICO 2000 LTD. Features & Benefits Compared to many other analytical methods, Ion-selective measurements are remarkably analyte -specific, very cost effective & suitable for continuous monitoring of time dependent changes in analyte concentration. ELIT Ion-Analysers incorporate the Latest Technology & enable the user to realise the full potential of Ion-Selective measurements. ELIT Analyser software ensures error-free data acquisition, storing & processing, and provides the user with reliable and clear guidance during the measuring process. ELIT Multi-channel Analysers connect up to 8 Electrodes to one computer. During Continuous Monitoring, the Sampling time can be varied over a wide range. An Averaging Algorithm smoothes unstable and noisy measurements. ELIT ISE/pH Analyser:- - Enables the detection limit to be significantly lowered by using three different algorithms to obtain optimal results in the non-linear range of the calibration curve. - Allows the user to select the most suitable method from: Direct Potentiometry; Standard-Addition; Sample-Addition.

11. NICO 2000 LTD. Worldwide Applications The ELIT Range of Ion-Analysers Water Quality Monitoring  pH, Calcium & various ions in surface water (rivers, lakes & ponds)  Ammonium in ground water  pH, Ammonium & Nitrate in fish tanks & fish ponds Pollution Control  pH of acid rain, soil, surface water  Contamination of surface water and ground water with ammonium and nitrate  Contamination of waste water with Cyanide, Cadmium, Mercury & Copper Food Quality Control  Nitrate & Nitrite in meat & vegetables  Chloride, Sodium, Nitrate & Nitrite in baby food  Fluoride & Calcium in milk & other milk products  Cadmium in fish Agriculture & Fishery  Soil & Fertilizer Analysis for Nitrate, Ammonium & Potassium to optimize the use of fertilizer (cost savings & highest yield)  Dissolved Oxygen and pH in Ponds for Fish Breeding Medical Diagnosis & Hygiene Control  Potassium in urine  Contamination Control for various Ions Research & Education  pH, Redox in Higher Education  All types of Ion Measurements & Continuous Monitoring in Schools and Colleges Pharmaceutical & Cosmetics Industry  Fluoride & pH of Tooth Paste  pH of Hair shampoo  Many other Ions determined in Pharmaceutical and Cosmetic Research & Development Waste Water Treatment  pH, Ammonium, Nitrate & other Ions used during processing Industrial Production  Salinity & pH of Boiler feed water  Cyanide in Plating baths  Process-specific Ions

12. NICO 2000 LTD. Hardware Details Solid all-ABS plastic enclosure ensures good resistance to corrosive spills and atmosphere. Plug in AC / DC Power Adapters with country-specific plugs. Optional small Battery Power Pack for use in the field. BNC connectors for Electrodes. 3.5mm connectors for Temperature sensors. 2mm connectors for external Reference electrodes (if required). Supplied with data cable and adapter for connection to 25-way or 9-way serial port on the computer (RS232). Optional special “active” cable for connecting to USB ports on the computer Stainless steel temperature sensors with optional Teflon coating. Electrode Amplifiers guarantee the best results with all types of ion-selective & pH electrodes (standard, special & micro). Powerful Microprocessor allows high resolution measurements & wide range of sampling times for continuous monitoring. Signal ground completely isolated, therefore no Ground Loops.

13. NICO 2000 LTD. Software Details State-of-the-art, User-friendly “Virtual Instrumentation” using the power of modern computing for operator convenience, efficiency and data security/protection. Storage of up 100,000 data points for one continuous measurement (ELIT 9804 & 9808). Easy to integrate into Laboratory Management Systems (LIMS). Acquired Data well presented in Graphical & Table format. Very Flexible 2D & 3D Graphical Data Display (ELIT 9804 & 9808) Online Data Display Selectable Smoothing of noisy measurements by selecting the Averaging number. Good suppression of 50 Hz & 60 Hz interference Easy-to-implement Communication Protocol if Ion-Analysers are connected to operating systems other than MS Windows

14. NICO 2000 LTD. Using the ISE / pH Ion- Analyser

15. NICO 2000 LTD. Calibration Procedure

16. NICO 2000 LTD. Calibration Curve

17. NICO 2000 LTD. Incremental ISE Determination

18. NICO 2000 LTD. Typical Graphical display from 4 or 8-Channel Analyser NB: Options Menu allows plotting of mV, Mol/L, pH or Temperature and any combination of Channels; individually or together. Graphic Tools enable wide range of options for graph styles, e.g. 3D, bar chart, labels, axes, ticks, etc.

19. NICO 2000 LTD. 3D Display of Data

20. NICO 2000 LTD. 3D Bar Chart Data Display

21. NICO 2000 LTD. Other NICO 2000 Products