1. Cells Interact with their Environment: The Extracellular Matrix

2. What is the extracellular matrix? Extra (E) cellular (C) matrix (M) Outside/outer of the cell surroundings within which something is contained The part of animal tissue (outside of the cells) that provides __________________________ to the cells contained within. What is the ECM composed of? _____________________: large polysaccharides with net negative charge, attracting __________ ions, such as ______, which then cause ___________ accumulation, _______________________ _________: collagen & elastin, which provide ______________ Cell adhesion proteins: fibronectin, which provides anchor points for cells to ____________ to.

3. Cell Interaction with the ECM (Migration) Steps: 1. Protrusion 2. Adhesion 3. Contraction 4. Release

4. How might a scientist study cell-ECM interactions? What characteristics might a synthetic ECM need What materials might have these desired qualities?

5. What is a polymer? Poly - mer - Definition: polymers are long-chain molecules consisting of repeating units (called: ______________) which are typically connected by ___________ chemical bonds. Synthetic Example Polyethylene (PE): CH2=CH2 CH2(CH2CH2)n Natural Example polysaccharide: Chitosan, randomly distributed D- glucosamine and N-acetyl-D-glucosamine units

6. Characterization of Polymers Skeletal structure: Primary structure: Homopolymer (1 monomer species) –A-A-A-A-A-A- Copolymer (2 or more monomer species) Random –A-B-A-A-B-A-B Block –A-A-A-B-B-B- Alternating –A-B-A-B-A-B Linear Branched Free Crosslinked

7. Polymer Usage as a Biomaterial What is a biomaterial? Examples:

8. Carbon Ring Numbering