1. Ecological Sampling

2. Why Do We Sample? Determine presence and/or abundance Monitor population fluctuations Assess ‘ecological damage’ Assess quality of habitat Assess population responses

3. What Do We Sample? Physical Environment –Temperature, DO, pH, salinity, clarity, flow, sediment Biotic Environment –All living things

4. Physical Habitat Temperature –Mercury thermometer –Electronic thermometer –Long-term thermometers Dissolved Oxygen –Winkler method (titration) –DO meter (electrode) pH –Litmus paper –pH meter (electrode) Salinity –Salinity Meter YSI 550A DO Meter w/12' cable

5. Water Clarity Secchi Disk –Disk is attached to a calibrated rope. The disk is lowered into the water until the white parts can no longer be seen. Secchi disk depth is then recorded and serves as the waters transparency index. The clearer the water, the greater the secchi disk depth. Secchi Disk

6. Current Velocity (flow) Floating-orange method. –Put an orange (or something else that floats just below the water surface) and measure the time it takes it to float across a known distance. Odometer-type flow meter –Number of revolutions the propeller makes for a given time is calibrated to flow velocity.

7. Sediment Sediment size is important to many aquatic organisms. Sieve’s are used to separate and grade sediment samples. –Percent of each size grade can be determined

8. Water Sample Water and plankton from various depths can be collected. A trigger mechanism is used to close the sampler. –Sample is then brought back to the surface

9. Small Mammals Mouse/rat Traps –Fatal Pit Falls –Bucket is placed in the ground –Sometimes have ‘leads’ to the buckets Live traps –Havahart –Sherman Spot-light Sherman trapHavahart trap

10. Birds Stick-under-the-box method Bird-trap –Works like a minnow trap Mist net –Captures birds in flight Rocket net –Uses a propellant to throw a net over birds

11. Terrestrial Insects Sticky paper –flies Baited Traps –Fire ants Nets –butterflies Foggers –Collect insects from tree canopies

12. Aquatic Insects Drift Net –Place net in flowing water Kick Net –‘Kick’ sediment upstream from block net and the flow will wash them into the net –Wash bucket Serber or Hess Sampler –Stir up known area of sediment –Animals are collected by a catch net Multi-plate Sampler –Become colonized

13. Crawfish and Crab Traps

14. Fish Larvae Light Traps –Larvae are attracted to the light Ichthyoplankton nets –Can be towed at various depths –Fish collect at the ‘cod’end

15. Fish Lift net –Net is placed down, and after a set amount of time it is quickly lifted Pop-net –Similar to a lift net, but floats are attached to a framed net. –Operated by a trigger mechanism Throw net –A net attached to a heavy frame is thrown and every thing inside is netted out Throw net Pop-net Lift net

16. Minnow trap Usually use bait to attract small fish –Light is used sometimes as an attractant

17. Fish Electrofishing –Electricity is put into the water –Fish are temporarily stunned and usually swim towards the electricity source –Usually non-fatal but may cause some damage

18. Fish Gill Net –Gill nets resemble tennis nets –Fish can not swim completely through the net and get caught –Gill nets are size selective (based on mesh size) Square Mesh Bar mesh Stretch mesh

19. Fish Trammel Net –Three panels: two large mesh on the outside and a small mesh on the inside –Fish swim through the outer mesh, pushes the small mesh through the other side and becomes entangeled

20. Hoop nets (and other similar nets) can have bait or not. Fyke nets have leads to help guide fish to the net.

21. Seine Seines are nets that are pulled through shallow water to catch fish.

22. Purse Seine Used to encircle entire schools of fish –Usually involves a spotter plane and a second boat

23. Trotline (longline) A series of baited drop lines connected to a main line. Can be deployed by tying one end to the bank and tying the other end with a heavy weight.

24. Shrimp (or fish) Trawl Net pulled behind a boat along the bottom –Either a beam or otter boards keep the net open

25. Tagging Individuals Coded Wire Tags –Microwire that has a unique label –Magnetic wand detects the tag –Tag retention should be determined T-Bar tags –Can be individually numbered –External tag PIT tags (Passive Integrated Transponders) –Wand induces the tag to transmit, individual number is displayed

26. Other Tagging Methods Toe clip –Amphibian and reptile –Clip of one or more toes to identify individuals Bird Band –Place a metal band on a bird leg –Generally has identification information