1. Événement Parlons Affaires Photonics North 2014, Montréal, 29 mai

2. Technologies originating from the research results of Prof. Denis Boudreau (COPL, Université Laval) Simple, fast and cost-effective tools for biodiagnostics and medical research Novel nanobiosensors Presented by Martin Castonguay, SOVAR Parlons Affaires Photonics North 2014, Montréal, 29 mai

3. Technology: “what and why ?” P lasmonic fluorescent core-shell nanoparticles 3 Silver core (~50 nm dia.) Silica shell (~3 nm th.)Molecular probe (no signal) Target molecule captured by molecular probe (strong signal) Silica surface for easy immobilization of biomolecules Silver core for enhanced fluorescence Strong signal Resistance to photobleaching Excellent signal/noise Versatility Molecular probe for specificity Parlons Affaires Photonics North 2014, Montréal, 29 mai

4. Application example #1: PCR-free genotyping Phenotyping and immunological methods are gradually being replaced by genotyping methods Genotyping methods utilizing PCR amplification of genetic material are costly, complex and time-consuming PCR-free cost-effective and timely genotyping tools would increase time-to-answer and decrease complexity and costs 4 Parlons Affaires Photonics North 2014, Montréal, 29 mai

5. Technological advantages DNA sensing based on fluorescent nanoparticles and a polymeric hybridization transducer 5 Sensitive:1000 copies, vs. 10 copies for PCR ~10M copies are available from 1 ml of blood Rapid: result available <10 min after mixing Simple sample preparation AND no amplification required Low cost:straightforward fabrication process and readily available instrumentation for detection Parlons Affaires Photonics North 2014, Montréal, 29 mai

6. Proof of principle SRY and Dombrock genes were detected directly from DNA extracted from human blood cells 6 Fluorescence signal (a.u.) Male donors Female donors Do a (+) Fluorescence signal (a.u.) Do a (-)Do b (+) Do b (-) SRY genotyping results for 10 non-amplified genomic DNA samples. 1 Dombrock (Do a /Do b ) genotyping results for 6 non-amplified genomic DNA samples. 2 Brouard D, Ratelle O et al. (2013) Analytical Methods 5(24) Unpublished data (2014). Parlons Affaires Photonics North 2014, Montréal, 29 mai

7. Preliminary evaluation of the cost per test 7 Batch sizeProjected cost per test Low scale, using research facilities and personel 6$ Large scale<1$ Parlons Affaires Photonics North 2014, Montréal, 29 mai

8. Application example #2: Functional cell imaging Parlons Affaires Photonics North 2014, Montréal, 29 mai 8 Adaptability to different functional fluorophores pH sensibility Calcium imagery Chloride Sodium, Potassium DNA probes Stable grafting on surfaces Non-invasive measurements Quantitative in vitro analyses Multi-elementary response Commercial microscopy hardware “Fluorescent nanosensors and uses thereof”, Canada/US provisional patent filed May 23, 2014.

9. 9 Parlons Affaires Photonics North 2014, Montréal, 29 mai Proof of principle Live extracellular pH measurements on calcified cardiac fibroblasts directly in the culture medium pH = 6.5 Substrate fluorescence Addition of therapeutic drug Adaptable for various studies Heart and lung cells (Na +, K +, pH) Cancer and tumors (pH, Cl -, Ca 2+ ) Neurons (Ca 2+, neurotransmitters)

10. IP protection 10 Patent 1: Polymer transducer Canada, US, Europe (UK, France, Germany), Australia and Japan Patent 2: Combination of polymer transducer to fluorescent reporter Canada, US, Europe (UK, France, Germany, Spain, Swiss), Australia and Japan Patent 3: Combination of polymer transducer and particles Canada, US. A patent application was just filed a week ago for the surface-grafted nanoparticles. Parlons Affaires Photonics North 2014, Montréal, 29 mai

11. Take home message ? 11 Fast time- to-answer Uses existing detection hardware Low-cost adaptable product International market Multiple applications Benefits for Canadians Newborn screening Cancer Pharmaco genomics Pathogen detection Parlons Affaires Photonics North 2014, Montréal, 29 mai Prof. Boudreau and his team are experts in the field of nanoparticles. If you are interested in functional nanoparticles for medical or other applications, there may well be a match between his science and your business needs ! denis.boudreau@chm.ulaval.ca