1. BLOOD AND BODY DEFENCE Dr. Amel Eassawi Dr. Abdelrahman Mustafa 1

2. HMIM 224 L5: BLOOD GROUPS AND TRANSFUSION 2

3. OBJECTIVES The students should be able to:  Differentiate between the different types of Blood groups and blood group system.  Explain blood typing and how it is used to avoid adverse reactions following blood transfusions.  Describe how blood reactions may occur between fetal and maternal tissues. 3

4. BLOOD GROUPS 4 In the year 1900, identification of the ABO blood antigen gene explained the observed blood type incompatibilities by Karl Landsteiner. Today there are 31 different genes known to contribute to the surface features of RBCs determining compatibility between blood types.

5. BLOOD GROUPS Major systems –ABO –Rhesus system (Rh) Minor –MN –P –Familial blood groups 5

6. BLOOD GROUPS Importance of Blood Groups: Transfusion of blood Association with disease Duodenal ulcers are more common in group O than in A or B Tumors of salivary glands, stomach and pancreas are more common in group A than in group O individuals. 6

7. 7 BLOOD GROUPS Terms to become familiar with: Agglutination – clumping of red blood cells in response to a reaction between an antibody and an antigen Antigens – a chemical that stimulates cells to produce antibodies Antibodies – a protein that reacts against a specific antigen

8. 8 BLOOD GROUPS ABO Blood Group: Based on the presence or absence of two major antigens on red blood cell membranes Antigen A Antigen B

9. 9 Type B blood Type AB bloodType O blood Red blood cell Anti-B antibody Antigen A Anti-A antibody Anti-B antibody Red blood cell Antigen A Antigen B Red blood cell Anti-A antibody Antigen B Type A blood

10. Antigens and antibodies of the ABO blood group Blood TypeAntigenAntibody AAAnti B BBAnti A ABA and BNeither Anti A nor Anti B ONeither A nor BBoth Anti A and Anti B 10

11. Genotype of ABO blood groups Blood GroupPossible Genotype(s) AAA, AO BBB, BO AB OOO 11

12. BLOOD GROUPS Landsteiner’s law: 1.If an agglutinogen (antigen) is present on the RBCs the corresponding agglutinin (anti body) must be absent in the plasma 2.If an agglutinogen (antigen) is absent on the RBCs the corresponding agglutinin (anti body) must be present in the plasma 12

13. 13 RH BLOOD GROUP The Rh blood group was named for the rhesus monkey The group includes several Rh antigens or factors (Cc, Dd & Ee) Rh positive – presence of antigen D or other Rh antigens on the red blood cell membranes. Rh negative – lack of these antigens. The seriousness of the Rh blood group is evident in a fetus that develops the condition erythroblastosis fetalis or hemolytic disease of the newborn.

14. ERYTHROBLSTOSIS FETALIS 14

15. ERYTHROBLASTOSIS FETALIS Clinical Features: –Edema –Jaundice –Anemia –Enlarged Liver & Spleen Treatment: –Exchange blood transfusion Prevention: –Rh immune globulin (RhIG) injections to the Rh – negative mother during pregnancy 15

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17. BLOOD GROUPS IN LOCAL POPULATION Blood GroupPercentage of Population O +48 % A +24 % B +17 % AB +4 % Rh Positive are about 93% (90-95%) O-4 % A-2% B-1 % AB-0.23% Rh negative are about 7% (5-10%) 17

18. PROFFERED AND PERMISSIBLE BLOOD TYPES FOR TRANSFUSION Blood Type of Recipient Preferred Blood Type of The Donor Permissible Blood Type of The Donor (In Extreme Emergency) AAO BBO AB A,B, O OONo alternate type 18

19. BLOOD TRANSFUSION Complications of Blood Transfusion : Fatal hemolytic reactions in mismatched reactions. Rapid hemolysis Jaundice Renal tubular damage Circulatory overload – if rapid transfusion Hemosiderosis – repeated blood transfusion Electrolyte disturbances Transmission of disease Hepatitis – B or C AIDS 19

20. REFERENCES  Human Physiology, Lauralee Sherwood, seventh edition.  Text book Physiology by Guyton &Hall,11 th edition.  Text book of Physiology by Linda S. Contanzo, third edition.  Physiology by Berne and Levy, sixth edition. 20