1. New Product Anouncement Cephalosporinase - Plates

2. Requirements for Plates ! Inactivation of a broad spectrum of cephalosporins Inactivation of residuals of disinfectants (especially contact plates)

3. ß-Lactam-Antibiotics Penicillins Ampicillin, Mezlocillin, Penicillin 1st Generation Cephalosporins: Cefazolin, Cefalotin, Cefalexim 2nd Generation Cephalosporins: Cefamandol, Cefuroxim, Cefoxitin (Cefomycin or Methoxa-Cephalosporine) 3rd Generation Cephalosporins: Cefotaxim, Ceftriaxon (Rocephin), Ceftazidim 4th Generation Cephalosporins: Cefquinom, Cefepim Carbapenems: Meropenem, Imipenem, Ertapenem

4. Activity of ß-Lactamase I ß-Lactamase I (= Penicillinase or Penase) hydrolyses the ß-Lactam ring of Penicillins Definition - Activity of Penicillinase: 1 Unit = Cleavage of 1 Micromol Penicillin G per minute at pH 7 and 25°C

5. Activity of ß-Lactamase II ß-Lactamase II (= Cephalosporinase or Cephase) hydrolyses the ß-lactam ring of Cephalosporins Definition - Activity of ß-Lactamase II: 1 Unit = Cleavage of 1 Micromol Cephalosporin C per minute at pH 7 and 25°C

6. Activity of different ß-Lactamase II Media - Tests using antibiotic discs -

7. Activity of different ß-Lactamase II Media - Tests using anitbiotic discs -

8. Summary of spectrum of efficacy for TSA Contact +LT +ß-Lactamase II (Article number 825) Good inactivation in antibiotic disc experiments: –Cefixim 5 µg /disc –Cefazolin 30 µg /disc –Meropenem 10 µg /disc –Cefotaxim 30 µg /disc –Ceftriaxon 30 µg /disc –Imipenem 10 µg /disc Moderate inactivation in antibiotic disc experiments: –Cefuroxim 30 µg /disc –Cefquinom 10 µg /disc Poor or missing inactivation in antibiotic disc experiments: –Cefepim 30 µg /disc –Cefoxitin 30 µg /disc

9. Developement of new Cephalosporinase with a broad inactivation spectrum LACTAMATOR TM

10. Inactivating Properties of Different ß- Lactamase II Media - Tests using Antibiotic Discs and S. aureus -

11. Inactivating Properties of Different ß- Lactamase II Media - Tests using Antibiotic Discs and K. rhizophila -

12. Summary of spectrum of efficacy for TSA Contact +LT +Cephase ICR+ (Article number 823) Good inactivation of the following antibiotics: –Penicillins: Ampicillin 25 µg, Mezlocillin 30 µg, Penicillin 10 µg –Cephalosporins 1st generation: Cefazolin 30 µg /disc –Cephalosporins 2nd generation: Cefuroxim 30 µg, Cefoxitin 30 µg (Cephamycin) –Cephalosporins 3rd generation: Cefotaxim 30 µg, Ceftriaxon 30 µg –Cephalosporins 4th generation: Cefepim 30 µg, Cefquinom 10 µg –Carbapenems: Ertapenem 10 µg, Imipenem, 10 µg, Meropenem 10 µg

13. New Products TSA Contact +LT + Cephase – ICR+ - article number 823 Product available for advance orders shelf life of 4 months completed in June 2009 TSA +LT + Cephase – ICR – article number 03085e Product available for advance orders shelf life of 4 months completed end of July 2009 LACTAMATOR TM LCT-5 (10 vials each with 50 IU Cephalosporinase and 500 IU Penicillinase, lyophilised) Product available in April 2009 LACTAMATOR TM LCT-20 (1 vial with 2 kIU Cephalosporinase and 20 kIU Penicillinase, lyophilised) Product available in April 2009