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Center for Homogeneous DNA Analysis - Software April 21, 2005.

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Presentation on theme: "Center for Homogeneous DNA Analysis - Software April 21, 2005."— Presentation transcript:

1 Center for Homogeneous DNA Analysis - Software April 21, 2005

2 Second Year Advances Background removal Melting curve calculators Automatic clustering and classification

3 Background removal Superior to baseline method Enables correct classification of unlabeled probe melting curves Makes HLA matching, repeat typing and sequencing more accurate Improves determination of thermodynamic parameters, and therefore all PCR design and analysis tools In use by ARUP Labs

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7 Melting curve calculators More informative than just Tm calculator Enhances design of full genotyping studies Improves determination of thermodynamic parameters

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10 Automatic clustering and classification algorithms Used in HLA identity testing, mutation scanning, and genotyping

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17 WWW availability Center for Homogeneous DNA analysis Homepage: www.dna.utah.eduwww.dna.utah.edu DNAWizards beta site by remote panels URL: DNAWizards.path.utah.eduDNAWizards.path.utah.edu Small amplicon primer design: SNPWizard Melting curve calculators: Melt1.0, Het1.0

18 The coming year Determine a complete set of Tm Parameters superior background removal realistic experimental conditions (dyes, salt) mismatched duplexes (synthetic) Genome wide primer promiscuity prevention Further develop user-friendly suite of programs Commercial launch of DNAWizards.com

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