1. Figure 1. Melting curve of SNP marker D1 of probe and PCR amplicon on LightScanner. SNA D1 is CA or CA deletion. It is very clear to see the genotype by the melting curve of unlabeled probe and the melting curve of amplicon also could distance the genotype too. So that the genotype could confirm by both way. A) Melting curve of unlabeled probe. B) Melting curve of amplicon. Figure 1AFigure 1B

2. Figure 2. Melting curve of SNP marker M1 of probe and PCR amplicon on LightScanner(n=16). SNA M1 is A or G. It is very clear to see the genotype by the melting curve of unlabeled probe and the melting curve of amplicon also could distance the genotype too. So that the genotype could confirm by both way. Figure2A. Melting curve of unlabeled probe. Figure2B. Melting curve of amplicon. AG AGAG A G Figure 2A Figure 2B

3. G::T G::C Figure 3. Human genomic wild type DNA (CFTR 542 G) and cystic fibrosis mutant DNA (CFTR 542 T) were mixed in different ratios. As mutation mixed with wild type increase, the G::C peak highs are decreased and G::T peak highs are increased. A) 10% increments of mutation from 0% to 100%. B) 2% increments of mutation from 0% to 10%. G::T G::C Figure 3A Figure 3B